Adsorption of human serum albumin onto gold: a combined electrochemical and ellipsometric study.

Human serum albumin adsorption onto gold surfaces was investigated by electrochemical and ellipsometric methods. Albumin adsorption onto gold was confirmed by the change of the open circuit potential of gold and by the ellipsometric parameter variation during albumin immobilization. In both experiments the parameters reached stable values within 10-15 min. The albumin adsorption layer thickness measured with the ellipsometer was about 1.5 nm. The adsorption of albumin under applied potential was also investigated and it was found that both positive and negative applied potential promote albumin adsorption. Changes in the optical parameters of bare gold and albumin adsorbed onto gold surface under applied potential were investigated with in situ ellipsometry. The similarity and reversibility of the optical changes showed that adsorbed albumin was stable on the gold surface under the applied potential range (-200-600 mV). The cyclic voltammograms of K3Fe(CN)6 on the modified gold surface showed that albumin could partly block the oxidation and reduction reaction.