AIM: To explore the role of hepatitis C virus (HCV) envelope protein 2 (E2) in the induction of apoptosis. METHODS: A carboxyterminal truncated E2 (E2-661) was transiently expressed in several cultured mammalian cell lines or stably expressed in Chinese hamster ovary (CHO) cell line. Cell proliferation was assessed by 3H thymidine uptake. Apoptosis was examined by Hoechst 33258 staining, flow cytometry and DNA fragmentation analysis. RESULTS: Reduced proliferation was readily observed in the E2-661 expressing cells. These cells manifested the typical features of apoptosis, including cell shrinkage, chromatin condensation and hypodiploid genomic DNA content. Similar apoptotic cell death was observed in an E2-661 stably expressing cell line. CONCLUSION: HCV E2 can induce apoptosis in cultured mammalian cells.
AIM: To explore the role of hepatitis C virus (HCV) envelope protein 2 (E2) in the induction of apoptosis. METHODS: A carboxyterminal truncated E2 (E2-661) was transiently expressed in several cultured mammalian cell lines or stably expressed in Chinese hamster ovary (CHO) cell line. Cell proliferation was assessed by 3H thymidine uptake. Apoptosis was examined by Hoechst 33258 staining, flow cytometry and DNA fragmentation analysis. RESULTS: Reduced proliferation was readily observed in the E2-661 expressing cells. These cells manifested the typical features of apoptosis, including cell shrinkage, chromatin condensation and hypodiploid genomic DNA content. Similar apoptotic cell death was observed in an E2-661 stably expressing cell line. CONCLUSION:HCVE2 can induce apoptosis in cultured mammalian cells.
Authors: Ednelza da Silva Graça Amoras; Samara Tatielle Monteiro Gomes; Felipe Bonfim Freitas; Bárbara Brasil Santana; Geraldo Ishak; Marialva Tereza Ferreira de Araújo; Sâmia Demachki; Simone Regina Souza da Silva Conde; Marluísa de Oliveira Guimarães Ishak; Ricardo Ishak; Antonio Carlos Rosário Vallinoto Journal: PLoS One Date: 2016-05-31 Impact factor: 3.240
Authors: Ann W N Auma; Carey L Shive; Alyssa Lange; Sofi Damjanovska; Corinne Kowal; Elizabeth Zebrowski; Pushpa Pandiyan; Brigid Wilson; Robert C Kalayjian; David H Canaday; Donald D Anthony Journal: Front Immunol Date: 2021-04-12 Impact factor: 7.561
Authors: Christoph P Berg; Stephan F Schlosser; Dorothee K H Neukirchen; Costa Papadakis; Michael Gregor; Sebastian Wesselborg; Gerburg M Stein Journal: Virol J Date: 2009-12-01 Impact factor: 4.099