AIM: Recombined plasmid pETNF-P16 was constructed to investigate its expression properties in esophageal squamous carcinoma cell line EC9706 induced by X-ray irradiation and the feasibility of gene-radiotherapy for esophageal carcinoma. METHODS: Recombined plasmid pETNF-P16 was constructed and transfected into EC9706 cells with lipofectamine. ELISA, Western blot, and immunocytochemistry were performed to determine the expression properties of pETNF-P16 in EC9706 after transfection induced by X-ray irradiation. RESULTS: Eukaryotic expression vector pETNF-P16 was successfully constructed and transfected into EC9706 cells. TNFalpha expressions were significantly increased in the transfected cells after different doses of X-ray irradiation than in those after 0Gy irradiation (1,192.330-2,026.518 pg/mL, P<0.05-0.01), and the TNFalpha expressions and P16 were significantly higher 6-48 h after 2 Gy X-ray irradiation (358.963-585.571 pg/mL, P<0.05-0.001). No P16 expression was detected in normal EC9706 cells. However, there was strong expression in the transfected and irradiation groups. CONCLUSION: X-ray irradiation induction could significantly enhance TNFalpha and P16 expression in EC9706 cells transfected with pETNF-P16 plasmid. These results may provide important experimental data and therapeutic potential for gene-radiotherapy of esophageal carcinoma.
AIM: Recombined plasmid pETNF-P16 was constructed to investigate its expression properties in esophageal squamous carcinoma cell line EC9706 induced by X-ray irradiation and the feasibility of gene-radiotherapy for esophageal carcinoma. METHODS: Recombined plasmid pETNF-P16 was constructed and transfected into EC9706 cells with lipofectamine. ELISA, Western blot, and immunocytochemistry were performed to determine the expression properties of pETNF-P16 in EC9706 after transfection induced by X-ray irradiation. RESULTS: Eukaryotic expression vector pETNF-P16 was successfully constructed and transfected into EC9706 cells. TNFalpha expressions were significantly increased in the transfected cells after different doses of X-ray irradiation than in those after 0Gy irradiation (1,192.330-2,026.518 pg/mL, P<0.05-0.01), and the TNFalpha expressions and P16 were significantly higher 6-48 h after 2 Gy X-ray irradiation (358.963-585.571 pg/mL, P<0.05-0.001). No P16 expression was detected in normal EC9706 cells. However, there was strong expression in the transfected and irradiation groups. CONCLUSION: X-ray irradiation induction could significantly enhance TNFalpha and P16 expression in EC9706 cells transfected with pETNF-P16 plasmid. These results may provide important experimental data and therapeutic potential for gene-radiotherapy of esophageal carcinoma.
Authors: N N Hanna; S Seetharam; H J Mauceri; M A Beckett; N T Jaskowiak; R M Salloum; D Hari; M Dhanabal; R Ramchandran; R Kalluri; V P Sukhatme; D W Kufe; R R Weichselbaum Journal: Cancer J Date: 2000 Sep-Oct Impact factor: 3.360
Authors: N Soufir; M F Avril; A Chompret; F Demenais; J Bombled; A Spatz; D Stoppa-Lyonnet; J Bénard; B Bressac-de Paillerets Journal: Hum Mol Genet Date: 1998-02 Impact factor: 6.150
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Authors: R R Weichselbaum; D E Hallahan; M A Beckett; H J Mauceri; H Lee; V P Sukhatme; D W Kufe Journal: Cancer Res Date: 1994-08-15 Impact factor: 12.701