Literature DB >> 15369864

Detection of Mycobacterium tuberculosis genotypic groups by a duplex real-time PCR targeting the katG and gyrA genes.

Laura Rindi1, Nicoletta Lari, Daniela Bonanni, Carlo Garzelli.   

Abstract

A duplex real-time PCR assay was developed for the assignment of Mycobacterium tuberculosis isolates to the three genotypic groups based on the katG463/gyrA95 polymorphism. The assay was as sensitive and specific as nucleotide sequencing and proved also able to detect unambiguously the isolate genotype in clinical specimens.

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Year:  2004        PMID： 15369864     DOI： 10.1016/j.mimet.2004.06.012

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  7 in total

1.  Genetic diversity, determined on the basis of katG463 and gyrA95 polymorphisms, Spoligotyping, and IS6110 typing, of Mycobacterium tuberculosis complex isolates from Italy.

Authors:  Nicoletta Lari; Laura Rindi; Christophe Sola; Daniela Bonanni; Nalin Rastogi; Enrico Tortoli; Carlo Garzelli
Journal:  J Clin Microbiol       Date:  2005-04       Impact factor: 5.948

2.  Visual format for detection of Mycobacterium tuberculosis and M. bovis in clinical samples using molecular beacons.

Authors:  Parameet Kumar; Kapili Nath; Bimba Rath; Manas K Sen; Potharuju Vishalakshi; Devender S Chauhan; Vishwa M Katoch; Sarman Singh; Sanjay Tyagi; Vishnubhatla Sreenivas; Hanumanthappa K Prasad
Journal:  J Mol Diagn       Date:  2009-08-06       Impact factor: 5.568

3.  Novel wide-range quantitative nested real-time PCR assay for Mycobacterium tuberculosis DNA: clinical application for diagnosis of tuberculous meningitis.

Authors:  Teruyuki Takahashi; Masato Tamura; Yukihiro Asami; Eiko Kitamura; Kosuke Saito; Tsukasa Suzuki; Sachiko Nonaka Takahashi; Koichi Matsumoto; Shigemasa Sawada; Eise Yokoyama; Toshiaki Takasu
Journal:  J Clin Microbiol       Date:  2008-03-12       Impact factor: 5.948

4.  Novel wide-range quantitative nested real-time PCR assay for Mycobacterium tuberculosis DNA: development and methodology.

Authors:  Teruyuki Takahashi; Masato Tamura; Yukihiro Asami; Eiko Kitamura; Kosuke Saito; Tsukasa Suzuki; Sachiko Nonaka Takahashi; Koichi Matsumoto; Shigemasa Sawada; Eise Yokoyama; Toshiaki Takasu
Journal:  J Clin Microbiol       Date:  2008-03-12       Impact factor: 5.948

5.  Novel technique of quantitative nested real-time PCR assay for Mycobacterium tuberculosis DNA.

Authors:  Teruyuki Takahashi; Tomohiro Nakayama
Journal:  J Clin Microbiol       Date:  2006-03       Impact factor: 5.948

Review 6.  Advances in real-time PCR: application to clinical laboratory diagnostics.

Authors:  Bernhard Kaltenboeck; Chengming Wang
Journal:  Adv Clin Chem       Date:  2005       Impact factor: 5.394

7.  Genomic variability of Mycobacterium tuberculosis strains of the Euro-American lineage based on large sequence deletions and 15-locus MIRU-VNTR polymorphism.

Authors:  Laura Rindi; Chiara Medici; Nicola Bimbi; Andrea Buzzigoli; Nicoletta Lari; Carlo Garzelli
Journal:  PLoS One       Date:  2014-09-08       Impact factor: 3.240
  7 in total

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