OBJECTIVE: Evaluation of the use of short probes as a detection system in real-time PCR. DESIGN AND METHOD: Comparison of results obtained with hybridization probes with and without locked nucleic acid (LNA) residues in the detection of fetal DNA in maternal serum. RESULTS: The use of chimeric LNA/DNA probes led to a slight but significantly higher level of sensitivity as well as a higher level of fluorescence signal. CONCLUSION: Chimeric LNA/DNA probes offer an interesting alternative detection method in real-time PCR.
OBJECTIVE: Evaluation of the use of short probes as a detection system in real-time PCR. DESIGN AND METHOD: Comparison of results obtained with hybridization probes with and without locked nucleic acid (LNA) residues in the detection of fetal DNA in maternal serum. RESULTS: The use of chimeric LNA/DNA probes led to a slight but significantly higher level of sensitivity as well as a higher level of fluorescence signal. CONCLUSION: Chimeric LNA/DNA probes offer an interesting alternative detection method in real-time PCR.
Authors: Mara R Diaz; James W Jacobson; Kelly D Goodwin; Sherry A Dunbar; Jack W Fell Journal: Limnol Oceanogr Methods Date: 2010-06-01 Impact factor: 2.634