The effect of thermal cycling on the bovine dentine shear bond strength of current adhesive systems.

The aim of this study was to evaluate the effect of thermal cycling on shear bond strength to bovine dentine of four current adhesive systems. Forty bovine incisors were ground flat with #600-grit silicon carbide paper to obtain superficial coronal dentine surfaces. Three one-bottle (etch and rinse) adhesive systems (Single Bond, Bond 1, One Step), and one self-etching primer adhesive system (Clearfil Liner Bond 2V) were evaluated. Dentine was conditioned and bonded with the adhesive systems tested according to the manufacturers' instructions. A cylindrical teflon mould (3 x 4 mm) was placed over the bonded area (to eliminate the bonded surface) and filled with composite resin. For each adhesive system the filling composite used was from the same manufacturer. Ten specimens were prepared for each material and all specimens were conditioned at 37 degrees C for 24 h. Five specimens of each material (half of the specimens) were thermocycled at 5000 cycles (at 5, 37, 55 and 37 degrees C) with a dwell time of 15 s and bonds were stressed in shear at a rate of 0.5 mm min(-1) until failure. Data were analysed by one-way anova and Student-Newman-Keuls rank test (alpha=0.05). Statistical analysis revealed that thermal cycling significantly (P < 0.05) reduced the bond strengths of all adhesive systems. Bond strengths prior to thermocycling were not significantly different (P > 0.05), except for One Step which revealed the lowest bond strength, and varied from 22.5 +/- 3.34 to 10.98 +/-0.87 MPa. After thermal cycling, the bond strengths ranged from 9.35 +/- 1.13 to 6.06 +/- 1.77 MPa. Failures after testing and prior to thermal cycle were most commonly adhesive failures between the bonding resin and dentine associated with partial cohesive failures in the adhesive resin (type 2). Adhesive failures between bonding resin and dentin (type 1) after thermocycling were most commonly seen.