Qingcai Wang1, Dimitris Horiatis, Jacek Pinski. 1. Division of Medical Oncology, Norris Comprehensive Cancer Center, University of Southern California, Los Angeles, California 90033, USA.
Abstract
BACKGROUND: The role of increased neuroendocrine (NE) differentiation in prostate cancer (PCA) is not well understood. Long-term exposure of the prostate cancer cell line LNCaP to high concentrations of the cytokine interleukin-6 (IL-6) results in permanent transdifferentiation of these cells into a NE phenotype. In this study, we evaluated the effect of IL-6-induced NE cells on the growth of the PCA cell lines LNCaP, PC-3, and DU-145 in vitro. RESULTS: Co-culture of NE cells with PCA cells significantly inhibited DNA synthesis in all three PCA cell lines by 30-90% compared to controls. NE cell co-culture resulted in an increased percentage of PCA cells arrested in the S-phase of cell cycle and PCA cell apoptosis. CONCLUSIONS: Our results imply that NE cells suppress the proliferation of surrounding PCA cells by release of inhibitory factors. 2004 Wiley-Liss, Inc.
BACKGROUND: The role of increased neuroendocrine (NE) differentiation in prostate cancer (PCA) is not well understood. Long-term exposure of the prostate cancer cell line LNCaP to high concentrations of the cytokine interleukin-6 (IL-6) results in permanent transdifferentiation of these cells into a NE phenotype. In this study, we evaluated the effect of IL-6-induced NE cells on the growth of the PCA cell lines LNCaP, PC-3, and DU-145 in vitro. RESULTS: Co-culture of NE cells with PCA cells significantly inhibited DNA synthesis in all three PCA cell lines by 30-90% compared to controls. NE cell co-culture resulted in an increased percentage of PCA cells arrested in the S-phase of cell cycle and PCA cell apoptosis. CONCLUSIONS: Our results imply that NE cells suppress the proliferation of surrounding PCA cells by release of inhibitory factors. 2004 Wiley-Liss, Inc.
Authors: Xuehong Deng; Bennett D Elzey; Jean M Poulson; Wallace B Morrison; Song-Chu Ko; Noah M Hahn; Timothy L Ratliff; Chang-Deng Hu Journal: Am J Cancer Res Date: 2011-08-18 Impact factor: 6.166
Authors: Tanya B Dorff; Bryan Goldman; Jacek K Pinski; Philip C Mack; Primo N Lara; Peter J Van Veldhuizen; David I Quinn; Nicholas J Vogelzang; Ian M Thompson; Maha H A Hussain Journal: Clin Cancer Res Date: 2010-05-18 Impact factor: 12.531