Literature DB >> 15365041

Detection and identification of Actinobacillus pleuropneumoniae serotypes 1, 2, and 8 by multiplex PCR.

Jennifer A Schuchert1, T J Inzana, Ø Angen, S Jessing.   

Abstract

Multiplex PCR assays were developed to identify Actinobacillus pleuropneumoniae serotypes 1, 2, and 8. Primers designed for the conserved capsular polysaccharide (CP) export region amplified a 489-bp DNA fragment from all serotypes. Primers specific to the CP biosynthesis regions of serotypes 1, 2, and 8 amplified fragments of 1.6 kb, 1.7 kb, and 970 bp from only their respective serotypes.

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Year:  2004        PMID： 15365041      PMCID： PMC516343          DOI： 10.1128/JCM.42.9.4344-4348.2004

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  20 in total

1.  Improved diagnostic PCR assay for Actinobacillus pleuropneumoniae based on the nucleotide sequence of an outer membrane lipoprotein.

Authors:  T Gram; P Ahrens
Journal:  J Clin Microbiol       Date:  1998-02       Impact factor: 5.948

2.  Identification and detection of Actinobacillus pleuropneumoniae by PCR based on the gene apxIVA.

Authors:  A Schaller; S P Djordjevic; G J Eamens; W A Forbes; R Kuhn; P Kuhnert; M Gottschalk; J Nicolet; J Frey
Journal:  Vet Microbiol       Date:  2001-03-02       Impact factor: 3.293

Review 3.  ABC transporters: bacterial exporters.

Authors:  M J Fath; R Kolter
Journal:  Microbiol Rev       Date:  1993-12

4.  Molecular cloning and analysis of genes for production of K5, K7, K12, and K92 capsular polysaccharides in Escherichia coli.

Authors:  I Roberts; R Mountford; N High; D Bitter-Suermann; K Jann; K Timmis; G Boulnois
Journal:  J Bacteriol       Date:  1986-12       Impact factor: 3.490

5.  Identification and characterization of a DNA region involved in the export of capsular polysaccharide by Actinobacillus pleuropneumoniae serotype 5a.

Authors:  C K Ward; T J Inzana
Journal:  Infect Immun       Date:  1997-06       Impact factor: 3.441

6.  Identification, genomic organization, and analysis of the group III capsular polysaccharide genes kpsD, kpsM, kpsT, and kpsE from an extraintestinal isolate of Escherichia coli (CP9, O4/K54/H5).

Authors:  T A Russo; S Wenderoth; U B Carlino; J M Merrick; A J Lesse
Journal:  J Bacteriol       Date:  1998-01       Impact factor: 3.490

7.  Evaluation of a PCR for detection of Actinobacillus pleuropneumoniae in mixed bacterial cultures from tonsils.

Authors:  T Gram; P Ahrens; J P Nielsen
Journal:  Vet Microbiol       Date:  1996-07       Impact factor: 3.293

8.  Evidence for a common molecular origin of the capsule gene loci in gram-negative bacteria expressing group II capsular polysaccharides.

Authors:  M Frosch; U Edwards; K Bousset; B Krausse; C Weisgerber
Journal:  Mol Microbiol       Date:  1991-05       Impact factor: 3.501

9.  Cloning and mutagenesis of a serotype-specific DNA region involved in encapsulation and virulence of Actinobacillus pleuropneumoniae serotype 5a: concomitant expression of serotype 5a and 1 capsular polysaccharides in recombinant A. pleuropneumoniae serotype 1.

Authors:  C K Ward; M L Lawrence; H P Veit; T J Inzana
Journal:  Infect Immun       Date:  1998-07       Impact factor: 3.441

10.  Evaluation of a multiplex PCR test for simultaneous identification and serotyping of Actinobacillus pleuropneumoniae serotypes 2, 5, and 6.

Authors:  Stine G Jessing; Øystein Angen; Tomas J Inzana
Journal:  J Clin Microbiol       Date:  2003-09       Impact factor: 5.948
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  13 in total

1.  Multiplex PCR that can distinguish between immunologically cross- reactive serovar 3, 6, and 8 Actinobacillus pleuropneumoniae strains.

Authors:  L Zhou; S C P Jones; Ø Angen; J T Bossé; J H E Nash; J Frey; R Zhou; H C Chen; J S Kroll; A N Rycroft; P R Langford
Journal:  J Clin Microbiol       Date:  2007-12-19       Impact factor: 5.948

2.  Use of miniaturized protein arrays for Escherichia coli O serotyping.

Authors:  Muna F Anjum; James D Tucker; Katherine A Sprigings; Martin J Woodward; Ralf Ehricht
Journal:  Clin Vaccine Immunol       Date:  2006-05

3.  Multiplex PCR assay for unequivocal differentiation of Actinobacillus pleuropneumoniae serovars 1 to 3, 5 to 8, 10, and 12.

Authors:  Janine T Bossé; Yanwen Li; Øystein Angen; Lucy A Weinert; Roy R Chaudhuri; Matthew T Holden; Susanna M Williamson; Duncan J Maskell; Alexander W Tucker; Brendan W Wren; Andrew N Rycroft; Paul R Langford
Journal:  J Clin Microbiol       Date:  2014-04-23       Impact factor: 5.948

4.  Genetic diversity of Actinobacillus pleuropneumoniae assessed by amplified fragment length polymorphism analysis.

Authors:  Branko Kokotovic; Øystein Angen
Journal:  J Clin Microbiol       Date:  2007-10-24       Impact factor: 5.948

5.  DNA microarray-based identification and typing of Actinobacillus pleuropneumoniae.

Authors:  GuoSheng Xiao; SanJie Cao; XiaoBo Huang; XinTian Wen
Journal:  Can J Vet Res       Date:  2009-07       Impact factor: 1.310

6.  Prevalence of Actinobacillus pleuropneumoniae serovars in England and Wales.

Authors:  C O'Neill; S C P Jones; J T Bossé; C M Watson; S M Williamson; A N Rycroft; J S Kroll; H M Hartley; P R Langford
Journal:  Vet Rec       Date:  2010-10-23       Impact factor: 2.695

7.  Development of a Multiplex PCR Assay for Rapid Molecular Serotyping of Haemophilus parasuis.

Authors:  Kate J Howell; Sarah E Peters; Jinhong Wang; Juan Hernandez-Garcia; Lucy A Weinert; Shi-Lu Luan; Roy R Chaudhuri; Øystein Angen; Virginia Aragon; Susanna M Williamson; Julian Parkhill; Paul R Langford; Andrew N Rycroft; Brendan W Wren; Duncan J Maskell; Alexander W Tucker
Journal:  J Clin Microbiol       Date:  2015-09-30       Impact factor: 5.948

8.  The genetic organization of the capsular polysaccharide biosynthesis region of Actinobacillus pleuropneumoniae serotype 14.

Authors:  Hiroya Ito
Journal:  J Vet Med Sci       Date:  2015-01-02       Impact factor: 1.267

9.  The genetic organization of the capsular polysaccharide biosynthesis region of Actinobacillus pleuropneumoniae serotype 15.

Authors:  Hiroya Ito; Masuo Sueyoshi
Journal:  J Vet Med Sci       Date:  2014-12-14       Impact factor: 1.267

10.  Characterization of the omlA gene from different serotypes of Actinobacillus pleuropneumoniae: A new insight into an old approach.

Authors:  Ciro César Rossi; Elza Fernandes de Araújo; Marisa Vieira de Queiroz; Denise Mara Soares Bazzolli
Journal:  Genet Mol Biol       Date:  2013-04-09       Impact factor: 1.771
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