Literature DB >> 15364458

Cloning of the varicella-zoster virus genome as an infectious bacterial artificial chromosome in Escherichia coli.

Kazuhiro Nagaike1, Yasuko Mori, Yasuyuki Gomi, Hironori Yoshii, Michiaki Takahashi, Markus Wagner, Ulrich Koszinowski, Koichi Yamanishi.   

Abstract

The complete genome of the varicella-zoster virus (VZV) Oka strain has been cloned as a bacterial artificial chromosome (BAC). Following electroporation into Escherichia coli (E. coli) strain DH10B, the VZV BAC was stably propagated over multiple generations of its host. Human embryonic lung (HEL) cells transfected with VZV BAC DNA recovered from DH10B showed cytopathic effect (CPE), and virus spread to neighbouring cells was observed. BAC vector sequences are flanked by loxP sites and, coinfection of the reconstituted virus, with a recombinant adenovirus expressing Cre recombinase removed the bacterial sequences. The resulting recombinant rV02 grew as well as the parental virus in HEL cells. The recombinant VZV will promote VZV research and increase use of the viral genome as an investigative tool.

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Year:  2004        PMID： 15364458     DOI： 10.1016/j.vaccine.2004.03.062

Source DB:  PubMed          Journal:  Vaccine        ISSN： 0264-410X            Impact factor:   3.641


  31 in total

1.  Cloning human herpes virus 6A genome into bacterial artificial chromosomes and study of DNA replication intermediates.

Authors:  Ronen Borenstein; Niza Frenkel
Journal:  Proc Natl Acad Sci U S A       Date:  2009-10-26       Impact factor: 11.205

Review 2.  Recent advances in cloning herpesviral genomes as infectious bacterial artificial chromosomes.

Authors:  Fuchun Zhou; Shou-Jiang Gao
Journal:  Cell Cycle       Date:  2011-02-01       Impact factor: 4.534

3.  Construction of an infectious rhesus rhadinovirus bacterial artificial chromosome for the analysis of Kaposi's sarcoma-associated herpesvirus-related disease development.

Authors:  Ryan D Estep; Michael F Powers; Bonnie K Yen; He Li; Scott W Wong
Journal:  J Virol       Date:  2007-01-10       Impact factor: 5.103

4.  Cloning the simian varicella virus genome in E. coli as an infectious bacterial artificial chromosome.

Authors:  Wayne L Gray; Fuchun Zhou; Juliane Noffke; B Karsten Tischer
Journal:  Arch Virol       Date:  2011-04-13       Impact factor: 2.574

5.  A herpes simplex virus 2 glycoprotein D mutant generated by bacterial artificial chromosome mutagenesis is severely impaired for infecting neuronal cells and infects only Vero cells expressing exogenous HVEM.

Authors:  Kening Wang; Justin D Kappel; Caleb Canders; Wilmer F Davila; Dean Sayre; Mayra Chavez; Lesley Pesnicak; Jeffrey I Cohen
Journal:  J Virol       Date:  2012-09-19       Impact factor: 5.103

6.  Varicella-zoster virus ORF49 functions in the efficient production of progeny virus through its interaction with essential tegument protein ORF44.

Authors:  Tomohiko Sadaoka; Satoshi Serada; Junko Kato; Mayuko Hayashi; Yasuyuki Gomi; Tetsuji Naka; Koichi Yamanishi; Yasuko Mori
Journal:  J Virol       Date:  2013-10-23       Impact factor: 5.103

7.  Varicella-zoster virus glycoprotein M homolog is glycosylated, is expressed on the viral envelope, and functions in virus cell-to-cell spread.

Authors:  Yoshiaki Yamagishi; Tomohiko Sadaoka; Hironori Yoshii; Pranee Somboonthum; Takayoshi Imazawa; Kazuhiro Nagaike; Keiichi Ozono; Koichi Yamanishi; Yasuko Mori
Journal:  J Virol       Date:  2007-10-31       Impact factor: 5.103

8.  Deletion in open reading frame 49 of varicella-zoster virus reduces virus growth in human malignant melanoma cells but not in human embryonic fibroblasts.

Authors:  Tomohiko Sadaoka; Hironori Yoshii; Takayoshi Imazawa; Koichi Yamanishi; Yasuko Mori
Journal:  J Virol       Date:  2007-09-12       Impact factor: 5.103

9.  A highly efficient protocol of generating and analyzing VZV ORF deletion mutants based on a newly developed luciferase VZV BAC system.

Authors:  Zhen Zhang; Ying Huang; Hua Zhu
Journal:  J Virol Methods       Date:  2008-01-22       Impact factor: 2.014

10.  A self-excisable infectious bacterial artificial chromosome clone of varicella-zoster virus allows analysis of the essential tegument protein encoded by ORF9.

Authors:  B Karsten Tischer; Benedikt B Kaufer; Marvin Sommer; Felix Wussow; Ann M Arvin; Nikolaus Osterrieder
Journal:  J Virol       Date:  2007-10-03       Impact factor: 5.103
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