BACKGROUND/AIMS: The role of NO (nitric oxide) in liver ischemia-reperfusion injury remains controversial, therefore the effect of sodium nitroprusside (SNP), as NO donor on ischemia-reperfusion injuries of the rat liver was studied. METHODOLOGY: Warm ischemia (68% of total liver) of the rat liver was induced for 30 min, followed by reperfusion for 60 min. A dose of 1 or 10 microg/kg/min SNP were continuously administered during the reperfusion period. We performed the following assessments: (i) hepatic microcirculation, (ii) nitrosyl-hemoglobin (Hb-NO, a product of interactions between hemoglobin and NO) levels, (iii) NO2-/NO3- (end products of NO oxidation) levels, and (iv) the serum lactate-dehydrogenase (LDH) levels. RESULTS: Hepatic microcirculation at 5 and 15 min after start of SNP infusion was significantly improved, compared with that in the control group. NO2-/NO3- and Hb-NO levels in both SNP groups were gradually elevated after reperfusion. Serum LDH levels at 60 min after start of reperfusion in both SNP groups were significantly lower than in the control group. CONCLUSIONS: In the early phase of the reperfusion period, SNP injected increased hepatic microcirculation and reduced hepatocyte damage, suggesting that the administration of SNP may help present ischemia-reperfusion injuries.
BACKGROUND/AIMS: The role of NO (nitric oxide) in liver ischemia-reperfusion injury remains controversial, therefore the effect of sodium nitroprusside (SNP), as NO donor on ischemia-reperfusion injuries of the rat liver was studied. METHODOLOGY: Warm ischemia (68% of total liver) of the rat liver was induced for 30 min, followed by reperfusion for 60 min. A dose of 1 or 10 microg/kg/min SNP were continuously administered during the reperfusion period. We performed the following assessments: (i) hepatic microcirculation, (ii) nitrosyl-hemoglobin (Hb-NO, a product of interactions between hemoglobin and NO) levels, (iii) NO2-/NO3- (end products of NO oxidation) levels, and (iv) the serum lactate-dehydrogenase (LDH) levels. RESULTS: Hepatic microcirculation at 5 and 15 min after start of SNP infusion was significantly improved, compared with that in the control group. NO2-/NO3- and Hb-NO levels in both SNP groups were gradually elevated after reperfusion. Serum LDH levels at 60 min after start of reperfusion in both SNP groups were significantly lower than in the control group. CONCLUSIONS: In the early phase of the reperfusion period, SNP injected increased hepatic microcirculation and reduced hepatocyte damage, suggesting that the administration of SNP may help present ischemia-reperfusion injuries.
Authors: Layton H Smith; John D Dixon; John R Stringham; Mesut Eren; Hassan Elokdah; Dave L Crandall; Kay Washington; Douglas E Vaughan Journal: Blood Date: 2005-09-13 Impact factor: 22.113
Authors: Gary Z Yu; Filip Istvanic; Xucai Chen; Mehdi Nouraie; Sruti Shiva; Adam C Straub; John J Pacella Journal: Ultrasound Med Biol Date: 2019-12-03 Impact factor: 2.998
Authors: Paul G Richardson; Vincent T Ho; Sergio Giralt; Sally Arai; Shin Mineishi; Corey Cutler; Joseph H Antin; Nicole Stavitzski; Dietger Niederwieser; Ernst Holler; Enric Carreras; Robert Soiffer Journal: Ther Adv Hematol Date: 2012-08