Yongqing Guo1, Xiaodong Zhao, Zhanquan Yang. 1. Department of Otolaryngology, First Clinical Academy of Medical College of Xiamen University, Xiamen, 361004, China.
Abstract
OBJECTIVE: In the present study, our aim was to develop a method to quantitative cell differentiation in cultures of human nasal epithelium (HNE) cells. METHOD: HNE cells were cultured on collagengel-coated membranes at an air-liquid interface (ALI) in hormone-and growth factor-supplemented medium. The percentage of the culture surface covered with ciliated cells was estimated using scanning electron microscope and image analysis. RESULT: In present study, if an ALI was not established and the cells were maintained in the submerged state, ciliated cell differentiation was pool, the average ciliated surface area was 0.3% when cultures were submerged for fourteen days. When HNE cells cultures in ALI, average ciliated surface area was 8.6%. CONCLUSION: Our study demonstrated increased ciliogenesis in ALI.
OBJECTIVE: In the present study, our aim was to develop a method to quantitative cell differentiation in cultures of human nasal epithelium (HNE) cells. METHOD: HNE cells were cultured on collagengel-coated membranes at an air-liquid interface (ALI) in hormone-and growth factor-supplemented medium. The percentage of the culture surface covered with ciliated cells was estimated using scanning electron microscope and image analysis. RESULT: In present study, if an ALI was not established and the cells were maintained in the submerged state, ciliated cell differentiation was pool, the average ciliated surface area was 0.3% when cultures were submerged for fourteen days. When HNE cells cultures in ALI, average ciliated surface area was 8.6%. CONCLUSION: Our study demonstrated increased ciliogenesis in ALI.