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Literature DB >> 15361659

Development of T-lymphocytes in mouse fetal thymus organ culture.

Tomoo Ueno¹, Cunlan Liu, Takeshi Nitta, Yousuke Takahama.

Abstract

Fetal thymus organ culture (FTOC) is a unique and powerful culture system that allows intrathymic T-lymphocyte development in vitro. T-cell development in FTOC well represents fetal thymocyte development in vivo. Here, we describe the basic method for FTOC as well as several related techniques, including the reconstitution of thymus lobes with T-lymphoid progenitor cells, high-oxygen submersion culture, time-lapse visualization of thymic emigration, reaggregation culture, and retrovirus-mediated gene transfer to developing thymocytes in FTOC.

Entities: Chemical Disease Species

Mesh：

Year: 2005 PMID： 15361659 DOI： 10.1385/1-59259-838-2:117

Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745

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Development of T-lymphocytes in mouse fetal thymus organ culture.

1. siRNA as a tool for investigating organogenesis: The pitfalls and the promises.

2. Diversity of TCRs on natural Foxp3+ T cells in mice lacking Aire expression.

3. Depletion and recovery of lymphoid subsets following morphine administration.

4. Gene expression profile of the third pharyngeal pouch reveals role of mesenchymal MafB in embryonic thymus development.

5. IAN family critically regulates survival and development of T lymphocytes.

6. Metabolite and thymocyte development defects in ADA-SCID mice receiving enzyme replacement therapy.

Review 7. Modeling of human T cell development in vitro as a read-out for hematopoietic stem cell multipotency.

8. Differential requirement of kindlin-3 for T cell progenitor homing to the non-vascularized and vascularized thymus.