Literature DB >> 15359726

Comparison of multiplexed techniques for detection of bacterial and viral proteins.

Rupa S Rao1, Steven R Visuri, Mary T McBride, Joanna S Albala, Dennis L Matthews, Matthew A Coleman.   

Abstract

Immobilized antibody microarrays were compared to the Luminex flow cytometry system that utilizes suspensions of polystyrene microbeads covalently coupled with capture antibodies. The two immunoassays were performed for comparison of reproducibility, limits of detection and dynamic range. The Luminex system showed lower limits of detection and increased dynamic range among samples whereas the protein microarrays could be more amenable to miniaturization. Both technologies were capable of sensitive multiplexed detection.

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Year:  2004        PMID: 15359726     DOI: 10.1021/pr034130t

Source DB:  PubMed          Journal:  J Proteome Res        ISSN: 1535-3893            Impact factor:   4.466


  6 in total

1.  Virus assay using antibody-functionalized peptide nanotubes.

Authors:  Robert I MacCuspie; Ipsita A Banerjee; Christophe Pejoux; Sanjay Gummalla; Howard S Mostowski; Philip R Krause; Hiroshi Matsui
Journal:  Soft Matter       Date:  2008-02-22       Impact factor: 3.679

2.  Development of a microsphere-based serologic multiplexed fluorescent immunoassay and a reverse transcriptase PCR assay to detect murine norovirus 1 infection in mice.

Authors:  Charlie C Hsu; Christiane E Wobus; Earl K Steffen; Lela K Riley; Robert S Livingston
Journal:  Clin Diagn Lab Immunol       Date:  2005-10

3.  Apparent thixotropic properties of saline/glycerol drops with biotinylated antibodies on streptavidin-coated glass slides: implications for bacterial capture on antibody microarrays.

Authors:  David M Albin; Andrew G Gehring; Sue A Reed; Shu-I Tu
Journal:  Sensors (Basel)       Date:  2009-02-16       Impact factor: 3.576

Review 4.  Immobilization techniques for microarray: challenges and applications.

Authors:  Satish Balasaheb Nimse; Keumsoo Song; Mukesh Digambar Sonawane; Danishmalik Rafiq Sayyed; Taisun Kim
Journal:  Sensors (Basel)       Date:  2014-11-25       Impact factor: 3.576

5.  An adaptable, portable microarray reader for biodetection.

Authors:  Deanna L Thompson; Francesca Pearson; Cynthia Thomas; Rupa Rao; Dennis Matthews; Joanna S Albala; Sebastian Wachsmann-Hogiu; Matthew A Coleman
Journal:  Sensors (Basel)       Date:  2009-04-14       Impact factor: 3.576

6.  Protein Chips for Detection of Salmonella spp. from Enrichment Culture.

Authors:  Palmiro Poltronieri; Fabio Cimaglia; Enrico De Lorenzis; Maurizio Chiesa; Valeria Mezzolla; Ida Barbara Reca
Journal:  Sensors (Basel)       Date:  2016-04-22       Impact factor: 3.576

  6 in total

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