| Literature DB >> 15358233 |
Masaki Oyama1, Mitsuo Wakasugi, Takashi Hama, Hatsuho Hashidume, Yasutaka Iwakami, Rika Imai, Sanae Hoshino, Hiroshi Morioka, Yasuhito Ishigaki, Osamu Nikaido, Tsukasa Matsunaga.
Abstract
Thymine glycol (Tg) is one of predominant oxidative DNA lesions caused by ionizing radiation and other oxidative stresses. Human NTH1 is a bifunctional enzyme with DNA glycosylase and AP lyase activities and removes Tg as the first step of base excision repair (BER). We have searched for the factors interacting with NTH1 by using a pull-down assay and found that GST-NTH1 fusion protein precipitates proliferating cell nuclear antigen (PCNA) and p53 as well as XPG from human cell-free extracts. GST-NTH1 also bound to recombinant FLAG-tagged XPG, PCNA, and (His)6-tagged p53 proteins, indicating direct protein-protein interaction between those proteins. Furthermore, His-p53 and FLAG-XPG, but not PCNA, stimulated the Tg DNA glycosylase/AP lyase activity of GST-NTH1 or NTH1. These results provide an insight into the positive regulation of BER reaction and also suggest a possible linkage between BER of Tg and other cellular mechanisms.Entities:
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Year: 2004 PMID： 15358233 DOI： 10.1016/j.bbrc.2004.06.136
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575