PURPOSE: Activin A is a member of the transforming growth factor beta superfamily and plays an important role in the differentiation of embryonic stem cells. We have reported previously that the expression of activin A is associated with lymph node metastasis in esophageal cancer, and our purpose in the current work is to clarify the molecular mechanism of the aggressive behavior of tumors that have high activin A expression. EXPERIMENTAL DESIGN: We have compared the gene expression profiles of human esophageal carcinoma cell lines that were stably transfected with activin beta A, which is a subunit of activin A, with those of control human esophageal carcinoma cell lines, using a cDNA microarray. RESULTS: We found that the expression level of neuronal cadherin (N-cadherin) was higher in the transfectants than in the control cells. N-cadherin was located on the cell surface of the transfectants, irrespective of the expression of epithelial cadherin (E-cadherin), and the expression of N-cadherin mRNA was significantly associated with that of activin beta A mRNA in clinical samples of esophageal carcinoma (n = 51; r = 0.855). A clinicopathologic analysis suggested that expression of N-cadherin mRNA was associated with the depth of tumor wall invasion, and a group of patients with high expression of N-cadherin mRNA showed a significantly poorer prognosis than a group of patients with low N-cadherin expression (P = 0.046). CONCLUSIONS: These results indicate that activin A might mediate the expression of N-cadherin and that this may be associated with depth of invasion and poor prognosis.
PURPOSE: Activin A is a member of the transforming growth factor beta superfamily and plays an important role in the differentiation of embryonic stem cells. We have reported previously that the expression of activin A is associated with lymph node metastasis in esophageal cancer, and our purpose in the current work is to clarify the molecular mechanism of the aggressive behavior of tumors that have high activin A expression. EXPERIMENTAL DESIGN: We have compared the gene expression profiles of humanesophageal carcinoma cell lines that were stably transfected with activin beta A, which is a subunit of activin A, with those of control humanesophageal carcinoma cell lines, using a cDNA microarray. RESULTS: We found that the expression level of neuronal cadherin (N-cadherin) was higher in the transfectants than in the control cells. N-cadherin was located on the cell surface of the transfectants, irrespective of the expression of epithelial cadherin (E-cadherin), and the expression of N-cadherin mRNA was significantly associated with that of activin beta A mRNA in clinical samples of esophageal carcinoma (n = 51; r = 0.855). A clinicopathologic analysis suggested that expression of N-cadherin mRNA was associated with the depth of tumor wall invasion, and a group of patients with high expression of N-cadherin mRNA showed a significantly poorer prognosis than a group of patients with low N-cadherin expression (P = 0.046). CONCLUSIONS: These results indicate that activin A might mediate the expression of N-cadherin and that this may be associated with depth of invasion and poor prognosis.
Authors: Holli A Loomans; Shanna A Arnold; Kate Hebron; Chase J Taylor; Andries Zijlstra; Claudia D Andl Journal: Am J Cancer Res Date: 2017-12-01 Impact factor: 6.166
Authors: Kerem M Senses; Mehdi Ghasemi; Muhammad W Akbar; Murat Isbilen; Anna L Fallacara; Shoshana Frankenburg; Silvia Schenone; Michal Lotem; Maurizio Botta; Ali O Gure Journal: Medchemcomm Date: 2016-10-27 Impact factor: 3.597
Authors: George S Hussey; Laura A Link; Andrew S Brown; Breege V Howley; Arindam Chaudhury; Philip H Howe Journal: PLoS One Date: 2012-12-20 Impact factor: 3.240
Authors: Walid Abushahba; Oyenike O Olabisi; Byeong-Seon Jeong; Rajeev K Boregowda; Yu Wen; Fang Liu; James S Goydos; Ahmed Lasfar; Karine A Cohen-Solal Journal: PLoS One Date: 2012-10-12 Impact factor: 3.240