OBJECTIVE: Assess the applicability of ion chromatography (IC) for human saliva studies. MATERIALS AND METHODS: The behaviour of an ion chromatograph (DX-100, Dionex) in the analysis of human saliva was examined in the following respects: calibration, reproducibility, recovery, and effect of pre-treatment conditions (dilution, temperature). The eluents used were 1.7 mM NaHCO3 + 1.8 mM Na2CO3 + 100 mg/L p-cyanophenol (2 mL/min) and 1.6 mM perfluorobutyric acid + 0.3 mM sodium hydroxide (0.3 mL/min) for inorganic and organic anions, respectively. RESULTS: The calibration curves were satisfactorily linear, but did not have unit slope (log-log plot) in the case of chloride, phosphate, sulphate and acetate. No significant effects were detected for centrifugation temperature, or between runs on the same occasion. CONCLUSION: IC appears to be a valuable technique well-suited to the efficient analysis of several salivary anions.
OBJECTIVE: Assess the applicability of ion chromatography (IC) for human saliva studies. MATERIALS AND METHODS: The behaviour of an ion chromatograph (DX-100, Dionex) in the analysis of human saliva was examined in the following respects: calibration, reproducibility, recovery, and effect of pre-treatment conditions (dilution, temperature). The eluents used were 1.7 mM NaHCO3 + 1.8 mM Na2CO3 + 100 mg/L p-cyanophenol (2 mL/min) and 1.6 mM perfluorobutyric acid + 0.3 mM sodium hydroxide (0.3 mL/min) for inorganic and organic anions, respectively. RESULTS: The calibration curves were satisfactorily linear, but did not have unit slope (log-log plot) in the case of chloride, phosphate, sulphate and acetate. No significant effects were detected for centrifugation temperature, or between runs on the same occasion. CONCLUSION: IC appears to be a valuable technique well-suited to the efficient analysis of several salivary anions.
Authors: Charlotte Duch Lynggaard; Christian Grønhøj; Robin Christensen; Anne Fischer-Nielsen; Jacob Melchiors; Lena Specht; Elo Andersen; Jann Mortensen; Peter Oturai; Gry Hoffmann Barfod; Eva Kannik Haastrup; Michael Møller-Hansen; Mandana Haack-Sørensen; Annette Ekblond; Jens Kastrup; Siri Beier Jensen; Christian von Buchwald Journal: Stem Cells Transl Med Date: 2022-05-27 Impact factor: 7.655