Literature DB >> 15352228

A peptide preconcentration approach for nano-high-performance liquid chromatography to diminish memory effects.

Heike Schaefer1, Jean-Pierre Chervet, Christian Bunse, Cornelia Joppich, Helmut E Meyer, Katrin Marcus.   

Abstract

An advanced method has been developed for the analysis of proteolytic digests of complex protein mixtures by reversed phase high-performance liquid chromatography coupled to mass spectrometry. The occurrence of memory effects was prevented by a parallel set of two precolumns employed for simultaneous separation and washing procedures. The system was tested extensively, and tryptic digests of three single proteins were analyzed. In addition, different solvent systems were evaluated for effective washing of the employed precolumns. Using the analytical strategy presented, a reliable identification of proteins in complex mixtures was obtained and not hampered by the occurrence of memory effects.

Mesh：

Substances：

Year:  2004        PMID： 15352228     DOI： 10.1002/pmic.200300801

Source DB:  PubMed          Journal:  Proteomics        ISSN： 1615-9853            Impact factor:   3.984


  9 in total

1.  The proteomic signature of insulin-resistant human skeletal muscle reveals increased glycolytic and decreased mitochondrial enzymes.

Authors:  J Giebelstein; G Poschmann; K Højlund; W Schechinger; J W Dietrich; K Levin; H Beck-Nielsen; K Podwojski; K Stühler; H E Meyer; H H Klein
Journal:  Diabetologia       Date:  2012-01-27       Impact factor: 10.122

2.  Identification of proteomic differences between squamous cell carcinoma of the lung and bronchial epithelium.

Authors:  Gereon Poschmann; Barbara Sitek; Bence Sipos; Anna Ulrich; Sebastian Wiese; Christian Stephan; Bettina Warscheid; Günter Klöppel; Ann Vander Borght; Frans C S Ramaekers; Helmut E Meyer; Kai Stühler
Journal:  Mol Cell Proteomics       Date:  2009-01-27       Impact factor: 5.911

3.  Detection of novel biomarkers of liver cirrhosis by proteomic analysis.

Authors:  Christian Mölleken; Barbara Sitek; Corinna Henkel; Gereon Poschmann; Bence Sipos; Sebastian Wiese; Bettina Warscheid; Christoph Broelsch; Markus Reiser; Scott L Friedman; Ida Tornøe; Anders Schlosser; Günter Klöppel; Wolff Schmiegel; Helmut E Meyer; Uffe Holmskov; Kai Stühler
Journal:  Hepatology       Date:  2009-04       Impact factor: 17.425

4.  Uncoupled responses of Smad4-deficient cancer cells to TNFalpha result in secretion of monomeric laminin-gamma2.

Authors:  Dirk Zboralski; Bettina Warscheid; Susanne Klein-Scory; M Bassel Malas; Heiko Becker; Miriam Böckmann; Helmut E Meyer; Wolff Schmiegel; Patricia Simon-Assmann; Irmgard Schwarte-Waldhoff
Journal:  Mol Cancer       Date:  2010-03-22       Impact factor: 27.401

5.  Immunoscreening of the extracellular proteome of colorectal cancer cells.

Authors:  Susanne Klein-Scory; Salwa Kübler; Hanna Diehl; Christina Eilert-Micus; Anke Reinacher-Schick; Kai Stühler; Bettina Warscheid; Helmut E Meyer; Wolff Schmiegel; Irmgard Schwarte-Waldhoff
Journal:  BMC Cancer       Date:  2010-02-25       Impact factor: 4.430

6.  A53T-alpha-synuclein-overexpression in the mouse nigrostriatal pathway leads to early increase of 14-3-3 epsilon and late increase of GFAP.

Authors:  Alexander Kurz; Caroline May; Oliver Schmidt; Thorsten Müller; Christian Stephan; Helmut E Meyer; Suzana Gispert; Georg Auburger; Katrin Marcus
Journal:  J Neural Transm (Vienna)       Date:  2011-09-30       Impact factor: 3.575

7.  Indirect protein quantification of drug-transforming enzymes using peptide group-specific immunoaffinity enrichment and mass spectrometry.

Authors:  Frederik Weiß; Anke Schnabel; Hannes Planatscher; Bart H J van den Berg; Bettina Serschnitzki; Andreas K Nuessler; Wolfgang E Thasler; Thomas S Weiss; Matthias Reuss; Dieter Stoll; Markus F Templin; Thomas O Joos; Katrin Marcus; Oliver Poetz
Journal:  Sci Rep       Date:  2015-03-04       Impact factor: 4.379

8.  Redox stress proteins are involved in adaptation response of the hyperthermoacidophilic archaeon Sulfolobus solfataricus to nickel challenge.

Authors:  Anna M Salzano; Ferdinando Febbraio; Tiziana Farias; Giovanni P Cetrangolo; Roberto Nucci; Andrea Scaloni; Giuseppe Manco
Journal:  Microb Cell Fact       Date:  2007-08-12       Impact factor: 5.328

9.  Mdm38 interacts with ribosomes and is a component of the mitochondrial protein export machinery.

Authors:  Ann E Frazier; Rebecca D Taylor; David U Mick; Bettina Warscheid; Nadine Stoepel; Helmut E Meyer; Michael T Ryan; Bernard Guiard; Peter Rehling
Journal:  J Cell Biol       Date:  2006-02-13       Impact factor: 10.539
  9 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.