Determination of dopamine by flow-injection analysis coupled with luminol-hexacyanoferrate(III) chemiluminescence detection.

A novel flow-injection method (FIA) for the determination of dopamine based on the inhibition of the intensity of chemiluminescence (CL) from luminol-hexacyanoferrate(III) system in basic medium is described. The present method allows the determination of dopamine over the range 30-100 microg l(-1) and 400-3000 microg l(-1). The relative standard deviation is 2.32% for 70 microg l(-1) dopamine and 1.22% for 1500 microg l(-1) dopamine (n = 20). The detection limit is 5 microg l(-1) with the sampling rate of 135 samples h(-1). This method has been applied for the determination of dopamine in commercial pharmaceutical injection samples. The results obtained by this method agreed with those by the official method.