Literature DB >> 15350732

Development of immunocapture reverse transcription loop-mediated isothermal amplification for the detection of tomato spotted wilt virus from chrysanthemum.

Shiro Fukuta1, Kazushi Ohishi, Keiko Yoshida, Yuko Mizukami, Akira Ishida, Michio Kanbe.   

Abstract

An immunocapture reverse transcription loop-mediated isothermal amplification (IC/RT-LAMP) was developed for the detection of tomato spotted wilt virus (TSWV) from chrysanthemum. This method enabled sensitive, reproducible and specific detection of TSWV from chrysanthemum plants. In the RT-LAMP method, TSWV genomic RNA could be amplified under isothermal (65 degrees C) conditions within 1 h. The resulting amplicons were detected by the measurement or observation of the turbidity of the reaction mixture without gel electrophoresis. IC/RT-LAMP was 100 times more sensitive than IC/RT-PCR.

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Year:  2004        PMID： 15350732     DOI： 10.1016/j.jviromet.2004.05.016

Source DB:  PubMed          Journal:  J Virol Methods        ISSN： 0166-0934            Impact factor:   2.014


  22 in total

1.  Detection of two exogenous genes in transgenic cattle by loop-mediated isothermal amplification.

Authors:  Shanli Zhai; Chuxin Liu; Qingde Zhang; Chenyu Tao; Bang Liu
Journal:  Transgenic Res       Date:  2012-06-09       Impact factor: 2.788

2.  Development of a visual detection method for Potato virus S by reverse transcription loop-mediated isothermal amplification.

Authors:  Ravinder Kumar; Priyanka Kaundal; Jeevalatha Arjunan; Sanjeev Sharma; S K Chakrabarti
Journal:  3 Biotech       Date:  2020-04-24       Impact factor: 2.406

3.  Loop-Mediated Isothermal Amplification Combined with PCR for Rapid Identification of the Ethiopian Fruit Fly (Diptera: Tephritidae).

Authors:  S Sabahi; L Fekrat; M Zakiaghl; G H Moravej
Journal:  Neotrop Entomol       Date:  2017-05-06       Impact factor: 1.434

4.  Loop-mediated isothermal amplification (LAMP) based detection of Colletotrichum falcatum causing red rot in sugarcane.

Authors:  Amaresh Chandra; Amber T Keizerweerd; Youxiong Que; Michael P Grisham
Journal:  Mol Biol Rep       Date:  2015-04-11       Impact factor: 2.316

5.  Detection of Puccinia kuehnii Causing Sugarcane Orange Rust with a Loop-Mediated Isothermal Amplification-Based Assay.

Authors:  Amaresh Chandra; Amber T Keizerweerd; Michael P Grisham
Journal:  Mol Biotechnol       Date:  2016-03       Impact factor: 2.695

6.  Rapid and sensitive detection of Pseudocercospora eumusae pathogen causing eumusae leaf spot disease of banana by loop-mediated isothermal amplification (LAMP) method.

Authors:  R Thangavelu; P Ganga Devi
Journal:  3 Biotech       Date:  2018-10-05       Impact factor: 2.406

7.  Rapid detection of wheat yellow mosaic virus by reverse transcription loop-mediated isothermal amplification.

Authors:  Zong-Ying Zhang; Xiao-Jun Liu; Da-Wei Li; Jia-Lin Yu; Cheng-Gui Han
Journal:  Virol J       Date:  2011-12-20       Impact factor: 4.099

8.  One-step detection of Bean pod mottle virus in soybean seeds by the reverse-transcription loop-mediated isothermal amplification.

Authors:  Qi-Wei Wei; Cui Yu; Shu-Ya Zhang; Cui-Yun Yang; Karwitha Miriam; Wen-Na Zhang; Dao-Long Dou; Xiao-Rong Tao
Journal:  Virol J       Date:  2012-09-07       Impact factor: 4.099

9.  Development of loop-mediated isothermal amplification for detection of Leifsonia xyli subsp. xyli in sugarcane.

Authors:  Jing Liu; Liping Xu; Jinlong Guo; Rukai Chen; Michael Paul Grisham; Youxiong Que
Journal:  Biomed Res Int       Date:  2013-04-23       Impact factor: 3.411

10.  Pepino mosaic virus genotype shift in North America and development of a loop-mediated isothermal amplification for rapid genotype identification.

Authors:  Kai-Shu Ling; Rugang Li; Michael Bledsoe
Journal:  Virol J       Date:  2013-04-12       Impact factor: 4.099
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