BACKGROUND: CD23 (FcepsilonRII) is expressed by intestinal epithelial cells (IEC) following allergic stimulation and increases the uptake of IgE/allergen complexes. The aim of this study was to further analyze the role of CD23 in the intraepithelial processing of food allergens during transepithelial transport. METHODS: Balb-C mice were sensitized intraperitoneally with horseradish peroxidase (HRP) or beta-lactoglobulin (beta-LG) in the presence of pertussis toxin. In control and sensitized mice, 3H-HRP, intact HRP, or 14C-beta-LG fluxes were measured across jejunal segments mounted in Ussing chambers, in the presence or absence of mucosal anti-CD23 antibodies. HPLC analysis of serosal buffer was performed to detect HRP- or beta-LG-derived radiolabelled metabolites generated during transepithelial transport. RESULTS: In HRP-sensitized mice, 3H-HRP fluxes and intact HRP fluxes (3,836 +/- 476 and 290 +/- 86 ng/h x cm2, respectively) were significantly increased compared to control mice (1,677 +/- 297 ng/h x cm2, p < 0.01, and 106 +/- 23 ng/h x cm2, p < 0.02, respectively). HPLC analysis indicated the presence of intact HRP in the serosal compartment already 10 min after addition of HRP to the mucosal compartment, a result not observed in the control mice. In the presence of anti-CD23 antibodies, intact HRP fluxes were significantly decreased (131 +/- 27 ng/h x cm2) compared to control values in sensitized mice (290 +/- 86 ng/h x cm2, p < 0.02), suggesting that CD23 is involved is this 'protected' transport pathway. A similar protection during intestinal transport was observed for beta-LG in beta-LG sensitized mice. CONCLUSIONS: These results confirm that CD23 is involved in the rapid transepithelial transport of intact allergens in sensitized animals, and indicate that CD23 opens a 'protected' transport pathway in IECs.
BACKGROUND:CD23 (FcepsilonRII) is expressed by intestinal epithelial cells (IEC) following allergic stimulation and increases the uptake of IgE/allergen complexes. The aim of this study was to further analyze the role of CD23 in the intraepithelial processing of food allergens during transepithelial transport. METHODS: Balb-C mice were sensitized intraperitoneally with horseradish peroxidase (HRP) or beta-lactoglobulin (beta-LG) in the presence of pertussis toxin. In control and sensitized mice, 3H-HRP, intact HRP, or 14C-beta-LG fluxes were measured across jejunal segments mounted in Ussing chambers, in the presence or absence of mucosal anti-CD23 antibodies. HPLC analysis of serosal buffer was performed to detect HRP- or beta-LG-derived radiolabelled metabolites generated during transepithelial transport. RESULTS: In HRP-sensitized mice, 3H-HRP fluxes and intact HRP fluxes (3,836 +/- 476 and 290 +/- 86 ng/h x cm2, respectively) were significantly increased compared to control mice (1,677 +/- 297 ng/h x cm2, p < 0.01, and 106 +/- 23 ng/h x cm2, p < 0.02, respectively). HPLC analysis indicated the presence of intact HRP in the serosal compartment already 10 min after addition of HRP to the mucosal compartment, a result not observed in the control mice. In the presence of anti-CD23 antibodies, intact HRP fluxes were significantly decreased (131 +/- 27 ng/h x cm2) compared to control values in sensitized mice (290 +/- 86 ng/h x cm2, p < 0.02), suggesting that CD23 is involved is this 'protected' transport pathway. A similar protection during intestinal transport was observed for beta-LG in beta-LG sensitized mice. CONCLUSIONS: These results confirm that CD23 is involved in the rapid transepithelial transport of intact allergens in sensitized animals, and indicate that CD23 opens a 'protected' transport pathway in IECs.
Authors: M Acharya; G Borland; A L Edkins; L M Maclellan; J Matheson; B W Ozanne; W Cushley Journal: Clin Exp Immunol Date: 2010-10 Impact factor: 4.330
Authors: J Abed; C Lebreton; G Champier; A Cuvillier; M Cogné; B Meresse; C Dugave; M Garfa-Traoré; B Corthésy; N Cerf-Bensussan; M Heyman Journal: Mucosal Immunol Date: 2013-07-10 Impact factor: 7.313
Authors: Eva Untersmayr; Giovanna Bises; Philipp Starkl; Charles L Bevins; Otto Scheiner; George Boltz-Nitulescu; Fritz Wrba; Erika Jensen-Jarolim Journal: PLoS One Date: 2010-02-02 Impact factor: 3.240
Authors: Lisa M Bartnikas; Michael F Gurish; Oliver T Burton; Sabine Leisten; Erin Janssen; Hans C Oettgen; Jacqueline Beaupré; Christopher N Lewis; K Frank Austen; Stephanie Schulte; Jason L Hornick; Raif S Geha; Michiko K Oyoshi Journal: J Allergy Clin Immunol Date: 2013-02 Impact factor: 10.793
Authors: Adam P Matson; Li Zhu; Elizabeth G Lingenheld; Craig M Schramm; Robert B Clark; Dawn M Selander; Roger S Thrall; Elena Breen; Lynn Puddington Journal: J Immunol Date: 2007-07-15 Impact factor: 5.422
Authors: Jolanda H M van Bilsen; Edyta Sienkiewicz-Szłapka; Daniel Lozano-Ojalvo; Linette E M Willemsen; Celia M Antunes; Elena Molina; Joost J Smit; Barbara Wróblewska; Harry J Wichers; Edward F Knol; Gregory S Ladics; Raymond H H Pieters; Sandra Denery-Papini; Yvonne M Vissers; Simona L Bavaro; Colette Larré; Kitty C M Verhoeckx; Erwin L Roggen Journal: Clin Transl Allergy Date: 2017-05-12 Impact factor: 5.871
Authors: E Jensen-Jarolim; H J Bax; R Bianchini; S Crescioli; T R Daniels-Wells; D Dombrowicz; E Fiebiger; H J Gould; S Irshad; J Janda; D H Josephs; F Levi-Schaffer; L O'Mahony; G Pellizzari; M L Penichet; F Redegeld; F Roth-Walter; J Singer; E Untersmayr; L Vangelista; S N Karagiannis Journal: Allergy Date: 2017-10-05 Impact factor: 13.146