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Literature DB >> 15345466

Production of minicellulosomes from Clostridium cellulovorans in Bacillus subtilis WB800.

Hee-Yeon Cho¹, Hideaki Yukawa, Masayuki Inui, Roy H Doi, Sui-Lam Wong.

Abstract

Two genes encoding EngB endoglucanase and mini-CbpA1 scaffolding protein of Clostridium cellulovorans were constructed and coexpressed in Bacillus subtilis WB800. The resulting minicellulosomes were isolated by gel filtration chromatography and characterized. Biochemical and immunological evidence indicated that fraction II contained minicellulosomes consisting of mini-CbpA1 and EngB. The in vivo synthesis of minicellulosomes suggests that it will be possible in the future to insert into B. subtilis cellulosomal genes that will allow growth on cellulosic materials and the production of various designer cellulosomes with specific functions.

Entities: Species

Mesh：

Substances：
DNA Primers

Year: 2004 PMID： 15345466 PMCID： PMC520898 DOI： 10.1128/AEM.70.9.5704-5707.2004

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

26 in total

Review 1. Cellulosomes from mesophilic bacteria.

Authors: Roy H Doi; Akihiko Kosugi; Koichiro Murashima; Yutaka Tamaru; Sung Ok Han
Journal: J Bacteriol Date: 2003-10 Impact factor: 3.490

2. Characterization of xylanolytic enzymes in Clostridium cellulovorans: expression of xylanase activity dependent on growth substrates.

Authors: A Kosugi; K Murashima; R H Doi
Journal: J Bacteriol Date: 2001-12 Impact factor: 3.490

3. Development of an inducible and enhancible expression and secretion system in Bacillus subtilis.

Authors: S L Wong
Journal: Gene Date: 1989-11-30 Impact factor: 3.688

4. The engL gene cluster of Clostridium cellulovorans contains a gene for cellulosomal manA.

Authors: Y Tamaru; R H Doi
Journal: J Bacteriol Date: 2000-01 Impact factor: 3.490

5. Heterologous production of Clostridium cellulovorans engB, using protease-deficient Bacillus subtilis, and preparation of active recombinant cellulosomes.

Authors: Koichiro Murashima; Chyi-Liang Chen; Akihiko Kosugi; Yutaka Tamaru; Roy H Doi; Sui-Lam Wong
Journal: J Bacteriol Date: 2002-01 Impact factor: 3.490

6. Three surface layer homology domains at the N terminus of the Clostridium cellulovorans major cellulosomal subunit EngE.

Authors: Y Tamaru; R H Doi
Journal: J Bacteriol Date: 1999-05 Impact factor: 3.490

Review 7. The Clostridium cellulovorans cellulosome: an enzyme complex with plant cell wall degrading activity.

Authors: R H Doi; Y Tamaru
Journal: Chem Rec Date: 2001 Impact factor: 6.771

8. Characterization of the CipA scaffolding protein and in vivo production of a minicellulosome in Clostridium acetobutylicum.

Authors: Fabrice Sabathé; Philippe Soucaille
Journal: J Bacteriol Date: 2003-02 Impact factor: 3.490

9. Nucleotide sequence and characteristics of endoglucanase gene engB from Clostridium cellulovorans.

Authors: F Foong; T Hamamoto; O Shoseyov; R H Doi
Journal: J Gen Microbiol Date: 1991-07

10. Engineering a Bacillus subtilis expression-secretion system with a strain deficient in six extracellular proteases.

Authors: X C Wu; W Lee; L Tran; S L Wong
Journal: J Bacteriol Date: 1991-08 Impact factor: 3.490

15 in total

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2. Profile of Roy H. Doi.

Authors: Nick Zagorski
Journal: Proc Natl Acad Sci U S A Date: 2007-05-29 Impact factor: 11.205

Review 3. Research progress and the biotechnological applications of multienzyme complex.

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Journal: Appl Microbiol Biotechnol Date: 2021-02-10 Impact factor: 4.813

4. Direct conversion of xylan to ethanol by recombinant Saccharomyces cerevisiae strains displaying an engineered minihemicellulosome.

Authors: Jie Sun; Fei Wen; Tong Si; Jian-He Xu; Huimin Zhao
Journal: Appl Environ Microbiol Date: 2012-03-23 Impact factor: 4.792

5. Enhanced microbial utilization of recalcitrant cellulose by an ex vivo cellulosome-microbe complex.

Authors: Chun You; Xiao-Zhou Zhang; Noppadon Sathitsuksanoh; Lee R Lynd; Y-H Percival Zhang
Journal: Appl Environ Microbiol Date: 2011-12-30 Impact factor: 4.792

6. Synthesis of Clostridium cellulovorans minicellulosomes by intercellular complementation.

Authors: Takamitsu Arai; Satoshi Matsuoka; Hee-Yeon Cho; Hideaki Yukawa; Masayuki Inui; Sui-Lam Wong; Roy H Doi
Journal: Proc Natl Acad Sci U S A Date: 2007-01-23 Impact factor: 11.205

7. Yeast surface display of trifunctional minicellulosomes for simultaneous saccharification and fermentation of cellulose to ethanol.

Authors: Fei Wen; Jie Sun; Huimin Zhao
Journal: Appl Environ Microbiol Date: 2009-12-18 Impact factor: 4.792

8. In vitro reconstitution of the complete Clostridium thermocellum cellulosome and synergistic activity on crystalline cellulose.

Authors: Jan Krauss; Vladimir V Zverlov; Wolfgang H Schwarz
Journal: Appl Environ Microbiol Date: 2012-04-20 Impact factor: 4.792

9. Heterologous production, assembly, and secretion of a minicellulosome by Clostridium acetobutylicum ATCC 824.

Authors: Florence Mingardon; Stéphanie Perret; Anne Bélaïch; Chantal Tardif; Jean-Pierre Bélaïch; Henri-Pierre Fierobe
Journal: Appl Environ Microbiol Date: 2005-03 Impact factor: 4.792

Review 10. The prospects of cellulase-producing bacteria for the bioconversion of lignocellulosic biomass.

Authors: Miranda Maki; Kam Tin Leung; Wensheng Qin
Journal: Int J Biol Sci Date: 2009-07-29 Impact factor: 6.580