Literature DB >> 15345463

Rapid estimation of numbers of fecal Bacteroidetes by use of a quantitative PCR assay for 16S rRNA genes.

Linda K Dick1, Katharine G Field.   

Abstract

Assessment of health risk associated with fecal pollution requires a reliable fecal indicator and a rapid quantification method. We report the development of a Taq nuclease assay for enumeration of 16S rRNA genes of Bacteroidetes. Sensitivity and correlation with standard fecal indicators provide experimental evidence for application of the assay in monitoring fecal pollution.

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Year:  2004        PMID： 15345463      PMCID： PMC520899          DOI： 10.1128/AEM.70.9.5695-5697.2004

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  11 in total

1.  Identification of nonpoint sources of fecal pollution in coastal waters by using host-specific 16S ribosomal DNA genetic markers from fecal anaerobes.

Authors:  A E Bernhard; K G Field
Journal:  Appl Environ Microbiol       Date:  2000-04       Impact factor: 4.792

2.  PCR bias in ecological analysis: a case study for quantitative Taq nuclease assays in analyses of microbial communities.

Authors:  S Becker; P Böger; R Oehlmann; A Ernst
Journal:  Appl Environ Microbiol       Date:  2000-11       Impact factor: 4.792

3.  A PCR assay To discriminate human and ruminant feces on the basis of host differences in Bacteroides-Prevotella genes encoding 16S rRNA.

Authors:  A E Bernhard; K G Field
Journal:  Appl Environ Microbiol       Date:  2000-10       Impact factor: 4.792

4.  Sources of Escherichia coli in a coastal subtropical environment.

Authors:  H M Solo-Gabriele; M A Wolfert; T R Desmarais; C J Palmer
Journal:  Appl Environ Microbiol       Date:  2000-01       Impact factor: 4.792

5.  Swimming-associated gastroenteritis and water quality.

Authors:  V J Cabelli; A P Dufour; L J McCabe; M A Levin
Journal:  Am J Epidemiol       Date:  1982-04       Impact factor: 4.897

6.  Influence of soil on fecal indicator organisms in a tidally influenced subtropical environment.

Authors:  Timothy R Desmarais; Helena M Solo-Gabriele; Carol J Palmer
Journal:  Appl Environ Microbiol       Date:  2002-03       Impact factor: 4.792

7.  An assessment of Bacteroides fragilis group organisms as indicators of human faecal pollution.

Authors:  K Allsop; D J Stickler
Journal:  J Appl Bacteriol       Date:  1985-01

8.  Survival and detection of Bacteroides spp., prospective indicator bacteria.

Authors:  L Fiksdal; J S Maki; S J LaCroix; J T Staley
Journal:  Appl Environ Microbiol       Date:  1985-01       Impact factor: 4.792

9.  Occurrence of Escherichia coli and enterococci in Cladophora (Chlorophyta) in nearshore water and beach sand of Lake Michigan.

Authors:  Richard L Whitman; Dawn A Shively; Heather Pawlik; Meredith B Nevers; Muruleedhara N Byappanahalli
Journal:  Appl Environ Microbiol       Date:  2003-08       Impact factor: 4.792

10.  Fecal indicator bacteria are abundant in wet sand at freshwater beaches.

Authors:  Elizabeth Wheeler Alm; Janice Burke; Anne Spain
Journal:  Water Res       Date:  2003-09       Impact factor: 11.236
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  46 in total

1.  Distribution of genetic marker concentrations for fecal indicator bacteria in sewage and animal feces.

Authors:  Catherine A Kelty; Manju Varma; Mano Sivaganesan; Richard A Haugland; Orin C Shanks
Journal:  Appl Environ Microbiol       Date:  2012-04-13       Impact factor: 4.792

2.  Unsuitability of quantitative Bacteroidales 16S rRNA gene assays for discerning fecal contamination of drinking water.

Authors:  Paul W J J van der Wielen; Gertjan Medema
Journal:  Appl Environ Microbiol       Date:  2010-05-28       Impact factor: 4.792

3.  Association of fecal indicator bacteria with human viruses and microbial source tracking markers at coastal beaches impacted by nonpoint source pollution.

Authors:  Shannon McQuaig; John Griffith; Valerie J Harwood
Journal:  Appl Environ Microbiol       Date:  2012-07-06       Impact factor: 4.792

4.  Field study of the composition of greywater and comparison of microbiological indicators of water quality in on-site systems.

Authors:  Margaret Leonard; Brent Gilpin; Beth Robson; Katrina Wall
Journal:  Environ Monit Assess       Date:  2016-07-19       Impact factor: 2.513

5.  Development of Bacteroides 16S rRNA gene TaqMan-based real-time PCR assays for estimation of total, human, and bovine fecal pollution in water.

Authors:  Alice Layton; Larry McKay; Dan Williams; Victoria Garrett; Randall Gentry; Gary Sayler
Journal:  Appl Environ Microbiol       Date:  2006-06       Impact factor: 4.792

6.  Development and applications of microbial ecogenomic indicators for monitoring water quality: report of a workshop assessing the state of the science, research needs and future directions.

Authors:  Richard Devereux; Parke Rublee; John H Paul; Katharine G Field; Jorge W Santo Domingo
Journal:  Environ Monit Assess       Date:  2006-05       Impact factor: 2.513

Review 7.  Performance, design, and analysis in microbial source tracking studies.

Authors:  Donald M Stoeckel; Valerie J Harwood
Journal:  Appl Environ Microbiol       Date:  2007-02-16       Impact factor: 4.792

8.  Evaluation of two library-independent microbial source tracking methods to identify sources of fecal contamination in French estuaries.

Authors:  Michèle Gourmelon; Marie Paule Caprais; Raphaël Ségura; Cécile Le Mennec; Solen Lozach; Jean Yves Piriou; Alain Rincé
Journal:  Appl Environ Microbiol       Date:  2007-06-08       Impact factor: 4.792

9.  Viral and Bacterial Fecal Indicators in Untreated Wastewater across the Contiguous United States Exhibit Geospatial Trends.

Authors:  Asja Korajkic; Brian McMinn; Michael P Herrmann; Mano Sivaganesan; Catherine A Kelty; Pat Clinton; Maliha S Nash; Orin C Shanks
Journal:  Appl Environ Microbiol       Date:  2020-04-01       Impact factor: 4.792

10.  Molecular detection of Campylobacter spp. and fecal indicator bacteria during the northern migration of sandhill cranes (Grus canadensis) at the central Platte River.

Authors:  Jingrang Lu; Hodon Ryu; Jason Vogel; Jorge Santo Domingo; Nicholas J Ashbolt
Journal:  Appl Environ Microbiol       Date:  2013-04-12       Impact factor: 4.792
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