Literature DB >> 15342605

Recombinant cyclophilins lack nuclease activity.

Angel Manteca1, Jesus Sanchez.   

Abstract

Several single-domain prokaryotic and eukaryotic cyclophilins have been identified as also being unspecific nucleases with a role in DNA degradation during the lytic processes that accompany bacterial cell death and eukaryotic apoptosis. Evidence is provided here that the supposed nuclease activity of human and bacterial recombinant cyclophilins is due to contamination of the proteins by the host Escherichia coli endonuclease and is not an intrinsic property of these proteins.

Entities:  

Mesh：

Substances：

Year:  2004        PMID： 15342605      PMCID： PMC515149          DOI： 10.1128/JB.186.18.6325-6326.2004

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  13 in total

Review 1.  Immunophilins: switched on protein binding domains?

Authors:  M T Ivery
Journal:  Med Res Rev       Date:  2000-11       Impact factor: 12.944

2.  Endonuclease-I-deficient and ribonuclease I-deficient Escherichia coli mutants.

Authors:  H Dürwald; H Hoffmann-Berling
Journal:  J Mol Biol       Date:  1968-07-14       Impact factor: 5.469

3.  E. coli host strains significantly affect the quality of small scale plasmid DNA preparations used for sequencing.

Authors:  R G Taylor; D C Walker; R R McInnes
Journal:  Nucleic Acids Res       Date:  1993-04-11       Impact factor: 16.971

4.  The periplasmic endonuclease I of Escherichia coli has amino-acid sequence homology to the extracellular DNases of Vibrio cholerae and Aeromonas hydrophila.

Authors:  M Jekel; W Wackernagel
Journal:  Gene       Date:  1995-02-27       Impact factor: 3.688

5.  A calcium-dependent nuclease from apoptotic rat thymocytes is homologous with cyclophilin. Recombinant cyclophilins A, B, and C have nuclease activity.

Authors:  J W Montague; M L Gaido; C Frye; J A Cidlowski
Journal:  J Biol Chem       Date:  1994-07-22       Impact factor: 5.157

6.  Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.

Authors:  C Yanisch-Perron; J Vieira; J Messing
Journal:  Gene       Date:  1985       Impact factor: 3.688

7.  Purification, characterization, and role of nucleases and serine proteases in Streptomyces differentiation. Analogies with the biochemical processes described in late steps of eukaryotic apoptosis.

Authors:  R G Nicieza; J Huergo; B A Connolly; J Sanchez
Journal:  J Biol Chem       Date:  1999-07-16       Impact factor: 5.157

8.  The hsp70 chaperone DnaK is a secondary amide peptide bond cis-trans isomerase.

Authors:  Cordelia Schiene-Fischer; Judith Habazettl; Franz X Schmid; Gunter Fischer
Journal:  Nat Struct Biol       Date:  2002-06

9.  Possible involvement of cyclophilin B and caspase-activated deoxyribonuclease in the induction of chromosomal DNA degradation in TCR-stimulated thymocytes.

Authors:  T Nagata; H Kishi; Q L Liu; T Yoshino; T Matsuda; Z X Jin; K Murayama; K Tsukada; A Muraguchi
Journal:  J Immunol       Date:  2000-10-15       Impact factor: 5.422

10.  Structure of human cyclophilin and its binding site for cyclosporin A determined by X-ray crystallography and NMR spectroscopy.

Authors:  J Kallen; C Spitzfaden; M G Zurini; G Wider; H Widmer; K Wüthrich; M D Walkinshaw
Journal:  Nature       Date:  1991-09-19       Impact factor: 49.962
View more
  2 in total

Review 1.  Apoptosis-inducing factor: structure, function, and redox regulation.

Authors:  Irina F Sevrioukova
Journal:  Antioxid Redox Signal       Date:  2011-03-10       Impact factor: 8.401

Review 2.  Molecular aspects of cyclophilins mediating therapeutic actions of their ligands.

Authors:  Andrzej Galat; Jacqueline Bua
Journal:  Cell Mol Life Sci       Date:  2010-07-04       Impact factor: 9.261
  2 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.