| Literature DB >> 15340973 |
Li Linxiang1, Yoshihiro Abe, Yukiko Nagasawa, Risa Kudo, Noriko Usui, Kazuhiro Imai, Tadahiko Mashino, Masataka Mochizuki, Naoki Miyata.
Abstract
An HPLC assay for hydroxyl radicals is described. The hydroxyl radical was trapped by terephthalic acid (non-fluorescent), and 2-hydroxyl terephthalic acid (fluorescent) was quantitated by HPLC-fluorescence detection. At a terephthalic acid concentration of 4.25 mmol/L, the hydroxyl radical formed in the Fenton reaction was successfully assayed in the concentration range of hydrogen peroxide of 2.5-50 micro mol/L, where the concentration of Fe(II) was 50 micro mol/L. The fluorescence of 2-hydroxy terephthalate was stable at 24 h, and its detection limit by this method was 5 nmol/L (100 fmol).Entities:
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Year: 2004 PMID： 15340973 DOI： 10.1002/bmc.339
Source DB: PubMed Journal: Biomed Chromatogr ISSN： 0269-3879 Impact factor: 1.902