Yu Guo1, Mi-fang Liang, Sheng-li Bi. 1. Laboratory of Hepatitis, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
Abstract
BACKGROUND: To investigate preparation of human-derived anti-HBs IgG antibody. METHODS: mRNA was extracted from lymphocytes of a volunteer with high level antibody to HbsAg. Fab phage display library was constructed. Purified HBsAg was used to screen the Fab. Eukaryotic expression vector was constructed and transfected into sf9 cells, then the supernatant was purified. The expression of anti-HBs human IgG was tested with blocking ELISA and immunofluorescent test. RESULTS: A whole human anti-HBs IgG molecule was obtained, the antibody reacts with HBsAg "a" determinant. CONCLUSION: It can be concluded preliminarily that this antibody has neutralizing characteristic against HbsAg.
BACKGROUND: To investigate preparation of human-derived anti-HBs IgG antibody. METHODS: mRNA was extracted from lymphocytes of a volunteer with high level antibody to HbsAg. Fab phage display library was constructed. Purified HBsAg was used to screen the Fab. Eukaryotic expression vector was constructed and transfected into sf9 cells, then the supernatant was purified. The expression of anti-HBs human IgG was tested with blocking ELISA and immunofluorescent test. RESULTS: A whole human anti-HBs IgG molecule was obtained, the antibody reacts with HBsAg "a" determinant. CONCLUSION: It can be concluded preliminarily that this antibody has neutralizing characteristic against HbsAg.
Authors: Ran Zhang; Dan Cui; Hong Wang; Chuan Li; Xin Yao; Yaofeng Zhao; Mifang Liang; Ning Li Journal: Transgenic Res Date: 2012-10 Impact factor: 2.788