Literature DB >> 15339801

Physical detwinning of hemihedrally twinned hexagonal crystals of bacteriorhodopsin.

Rouslan Efremov1, Rouslan Moukhametzianov, Georg Büldt, Valentin Gordeliy.   

Abstract

Hexagonal crystals of the membrane protein bacteriorhodopsin of space group P6 3 grown in lipidic cubic phase are twinned hemihedrally. It was shown that slow changes of salt concentration in the mother liquor lead to a split of crystals so that the split parts preserved high diffraction quality. Analysis of diffraction data from split crystals by Yeates statistic and Britton plot showed that the split parts are free of twinning. It is concluded that crystals of bacteriorhodopsin are composed of several macroscopic twinning domains with sizes comparable to the original crystal. The appearance of twinning domains during crystal growth and the mechanism of splitting are discussed.

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Year:  2004        PMID： 15339801      PMCID： PMC1304826          DOI： 10.1529/biophysj.104.046573

Source DB:  PubMed          Journal:  Biophys J        ISSN： 0006-3495            Impact factor:   4.033


  18 in total

1.  Crystallization and 2.2 A resolution structure of R-phycoerythrin from Gracilaria chilensis: a case of perfect hemihedral twinning.

Authors:  C Contreras-Martel; J Martinez-Oyanedel; M Bunster; P Legrand; C Piras; X Vernede; J C Fontecilla-Camps
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2001-01

2.  On the molecular-replacement problem in the presence of merohedral twinning: structure of the N-terminal half-molecule of human lactoferrin.

Authors:  W A Breyer; R L Kingston; B F Anderson; E N Baker
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  1999-01-01

3.  Detection and characterization of merohedral twinning in two protein crystals: bacteriorhodopsin and p67(phox).

Authors:  Antoine Royant; Sylvestre Grizot; Richard Kahn; Hassan Belrhali; Franck Fieschi; Ehud M Landau; Eva Pebay-Peyroula
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2002-04-26

Review 4.  A lipid's eye view of membrane protein crystallization in mesophases.

Authors:  M Caffrey
Journal:  Curr Opin Struct Biol       Date:  2000-08       Impact factor: 6.809

5.  Detecting and overcoming crystal twinning.

Authors:  T O Yeates
Journal:  Methods Enzymol       Date:  1997       Impact factor: 1.600

6.  Simple statistics for intensity data from twinned specimens.

Authors:  T O Yeates
Journal:  Acta Crystallogr A       Date:  1988-03-01       Impact factor: 2.290

7.  Crystallographic structure of the K intermediate of bacteriorhodopsin: conservation of free energy after photoisomerization of the retinal.

Authors:  Brigitte Schobert; Jill Cupp-Vickery; Viktor Hornak; Steven Smith; Janos Lanyi
Journal:  J Mol Biol       Date:  2002-08-23       Impact factor: 5.469

8.  Crystallographic structure of the retinal and the protein after deprotonation of the Schiff base: the switch in the bacteriorhodopsin photocycle.

Authors:  Janos Lanyi; Brigitte Schobert
Journal:  J Mol Biol       Date:  2002-08-23       Impact factor: 5.469

9.  Proton transfer pathways in bacteriorhodopsin at 2.3 angstrom resolution.

Authors:  H Luecke; H T Richter; J K Lanyi
Journal:  Science       Date:  1998-06-19       Impact factor: 47.728

10.  Lipid patches in membrane protein oligomers: crystal structure of the bacteriorhodopsin-lipid complex.

Authors:  L Essen; R Siegert; W D Lehmann; D Oesterhelt
Journal:  Proc Natl Acad Sci U S A       Date:  1998-09-29       Impact factor: 11.205
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  1 in total

1.  Time-resolved microspectroscopy on a single crystal of bacteriorhodopsin reveals lattice-induced differences in the photocycle kinetics.

Authors:  R Efremov; V I Gordeliy; J Heberle; G Büldt
Journal:  Biophys J       Date:  2006-05-26       Impact factor: 4.033
  1 in total

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