AIM: To identify the molecular basis of the endothelial target for acetylcholine (ETA). METHODS: Proteomic methods were used to monitor changes in protein expression in the first 10 h following the stimulation of human coronary endothelial cells with carbachol 100 micromol/L. Thirty proteins showing the largest changes were identified by mass spectrometry. RESULTS: Based on analysis with Image Master 2-D Elite software, about 623 protein spots were detected in control cells and 825 protein spots in carbachol-treated cells, the matching rate was 68.1 %. Among all the detected spots, 39 were up-regulated and 29 were down-regulated, showing detectable changes varied from 1.7-3.8 folds. Forty one spots in the peptide mass fingerprints were successfully obtained. The most interesting feature was that all the four newly synthesized proteins belonged to the heat shock protein family. CONCLUSION: These identified proteins played key roles in the molecular mechanism of ETA.
AIM: To identify the molecular basis of the endothelial target for acetylcholine (ETA). METHODS: Proteomic methods were used to monitor changes in protein expression in the first 10 h following the stimulation of human coronary endothelial cells with carbachol 100 micromol/L. Thirty proteins showing the largest changes were identified by mass spectrometry. RESULTS: Based on analysis with Image Master 2-D Elite software, about 623 protein spots were detected in control cells and 825 protein spots in carbachol-treated cells, the matching rate was 68.1 %. Among all the detected spots, 39 were up-regulated and 29 were down-regulated, showing detectable changes varied from 1.7-3.8 folds. Forty one spots in the peptide mass fingerprints were successfully obtained. The most interesting feature was that all the four newly synthesized proteins belonged to the heat shock protein family. CONCLUSION: These identified proteins played key roles in the molecular mechanism of ETA.
Authors: Muna Affara; Benjamin Dunmore; Christopher Savoie; Seiya Imoto; Yoshinori Tamada; Hiromitsu Araki; D Stephen Charnock-Jones; Satoru Miyano; Cristin Print Journal: Philos Trans R Soc Lond B Biol Sci Date: 2007-08-29 Impact factor: 6.237
Authors: Mary Ricardo-Dukelow; Irena Kadiu; Wojciech Rozek; Joshua Schlautman; Yuri Persidsky; Pawel Ciborowski; Georgette D Kanmogne; Howard E Gendelman Journal: J Neuroimmunol Date: 2007-02-23 Impact factor: 3.478