Assessing wastewater toxicity to activated sludge: recent research and developments.

Toxicants in municipal sewage treatment plant (STP) influent wastewater may inhibit the biological activity of the activated sludge and cause treatment plant process upsets. Such process upsets may be avoided if influent wastewater is monitored for toxicity and protective actions are taken when toxicity is detected. A comprehensive review of the methods that can be used for assessing wastewater toxicity to biological treatment systems was conducted several years ago and the resultant report was published in 2000 by Water Environment Research Foundation (WERF). The WERF report also specified the criteria for influent wastewater toxicity monitoring methods and suggested research needs to be addressed. A significant amount of effort was made since the publication of the WERF report to develop new assays or devices and to improve existing ones. In this manuscript, recent research and developments in methods for assessing wastewater toxicity to activated sludge were reviewed. The literature indicates that bioluminescence- and respirometry-based methods received much attention in recent research. A comparison of the new/improved methods with the criteria described in the WERF report reveals that none of these methods has been shown to meet all the specified criteria. The present review also indicates that research efforts since 2000 have not fully taken into account the criteria for influent wastewater toxicity monitoring methods and have not addressed the research needs proposed in the WERF report.