Shi-rong Li1, Jian-yi Liu, Shu-xing Ji. 1. Department of Plastic and Aesthetic Surgery, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
Abstract
OBJECTIVE: To explore the effect of connective tissue growth factor on the pathogenesis of human hypertrophic scar. METHODS: Normal skin and hypertrophic scar fibroblasts were cultured in vitro. The collagen synthesis of fibroblasts were measured by H3-proline incorporation method. The expression of connective tissue growth factor protein and mRNA of fibroblasts were detected with immunocytochemistry staining and reverse transcription polymerase chain reaction methods. RESULTS: Compared with normal skin fibroblast, the collagen synthesis and the expression of connective tissue growth factor protein and mRNA in the hypertrophic scar fibroblast was higher (P < 0.01). CONCLUSION: Connective tissue growth factor may play an important role in promoting the fibrotic process of hypertrophic scar.
OBJECTIVE: To explore the effect of connective tissue growth factor on the pathogenesis of humanhypertrophic scar. METHODS: Normal skin and hypertrophic scar fibroblasts were cultured in vitro. The collagen synthesis of fibroblasts were measured by H3-proline incorporation method. The expression of connective tissue growth factor protein and mRNA of fibroblasts were detected with immunocytochemistry staining and reverse transcription polymerase chain reaction methods. RESULTS: Compared with normal skin fibroblast, the collagen synthesis and the expression of connective tissue growth factor protein and mRNA in the hypertrophic scar fibroblast was higher (P < 0.01). CONCLUSION:Connective tissue growth factor may play an important role in promoting the fibrotic process of hypertrophic scar.
Authors: Mariam Y El-Hattab; Yoshiaki Nagumo; Francoise A Gourronc; Aloysius J Klingelhutz; James A Ankrum; Edward A Sander Journal: Sci Rep Date: 2020-06-24 Impact factor: 4.379