AIM: To explore the expression of heparanase mRNA and point mutation in hepatocellular carcinoma (HCC). METHODS: Reverse transcription polymerase chain reaction was used to measure the expression of heparanase mRNA in the primary tumor tissues and surrounding liver tissues of 33 HCC patients. T-A cloning and sequencing were used to detect whether there was any mutation in the amplified PCR products. RESULTS: The expression of heparanase mRNA was positive in 16 primary tumor tissues of HCC, and the positive rate was 48.5%, which was significantly higher than that in the surrounding liver parenchyma (P<0.01). The positive rate for heparanase gene in high-tendency to metastatic recurrence group (71.4%, 10/14) was obviously higher than that in low-tendency to metastatic recurrence group (31.6%, 6/19) (P = 0.023). The positive rate for heparanase gene in patients with metastatic recurrence during postoperative follow-up (78.6%, 11/14) was also significantly higher than that in those without metastatic recurrence (21.4%, 3/14) (P = 0.003). Sequence analysis of the HPA PCR products was made in 7 patients, and 2-point mutations were found in 4 patients, one of which was sense mutation, neither base insertion nor deletion was detected. The mutation rate was 57.1% (4/7). CONCLUSION: The expression rate of heparanase mRNA increases in HCC, and HPA mRNA may be one of the reliable markers for the metastatic activity gained by the liver tumor cells and could be used clinically in predicting metastatic recurrence of HCC. Point mutation may be one of the causes for enhanced heparanase mRNA expression.
AIM: To explore the expression of heparanase mRNA and point mutation in hepatocellular carcinoma (HCC). METHODS: Reverse transcription polymerase chain reaction was used to measure the expression of heparanase mRNA in the primary tumor tissues and surrounding liver tissues of 33 HCC patients. T-A cloning and sequencing were used to detect whether there was any mutation in the amplified PCR products. RESULTS: The expression of heparanase mRNA was positive in 16 primary tumor tissues of HCC, and the positive rate was 48.5%, which was significantly higher than that in the surrounding liver parenchyma (P<0.01). The positive rate for heparanase gene in high-tendency to metastatic recurrence group (71.4%, 10/14) was obviously higher than that in low-tendency to metastatic recurrence group (31.6%, 6/19) (P = 0.023). The positive rate for heparanase gene in patients with metastatic recurrence during postoperative follow-up (78.6%, 11/14) was also significantly higher than that in those without metastatic recurrence (21.4%, 3/14) (P = 0.003). Sequence analysis of the HPA PCR products was made in 7 patients, and 2-point mutations were found in 4 patients, one of which was sense mutation, neither base insertion nor deletion was detected. The mutation rate was 57.1% (4/7). CONCLUSION: The expression rate of heparanase mRNA increases in HCC, and HPA mRNA may be one of the reliable markers for the metastatic activity gained by the liver tumor cells and could be used clinically in predicting metastatic recurrence of HCC. Point mutation may be one of the causes for enhanced heparanase mRNA expression.
Authors: Hua-Quan Miao; Elizabeth Navarro; Sheetal Patel; David Sargent; Henry Koo; Hong Wan; Anadellys Plata; Qinwei Zhou; Dale Ludwig; Peter Bohlen; Paul Kussie Journal: Protein Expr Purif Date: 2002-12 Impact factor: 1.650
Authors: K Endo; U Maejara; H Baba; E Tokunaga; T Koga; Y Ikeda; Y Toh; S Kohnoe; T Okamura; M Nakajima; K Sugimachi Journal: Anticancer Res Date: 2001 Sep-Oct Impact factor: 2.480
Authors: Y Morita; Y Hayashi; Y Wang; T Kanamaru; S Suzuki; K Kawasaki; K Ohta; M Yamamoto; Y Saitoh; H Itoh; W F Doe Journal: Hepatology Date: 1997-04 Impact factor: 17.425
Authors: F I Staquicini; C R Moreira; F D Nascimento; I L Tersariol; H B Nader; C P Dietrich; J D Lopes Journal: Melanoma Res Date: 2003-02 Impact factor: 3.599
Authors: J Rohloff; J Zinke; K Schoppmeyer; A Tannapfel; H Witzigmann; J Mössner; C Wittekind; K Caca Journal: Br J Cancer Date: 2002-04-22 Impact factor: 7.640