Literature DB >> 15332624

Effects of serum and serum heat-inactivation on human bone derived osteoblast progenitor cells.

A Bruinink1, U Tobler, M Hälg, J Grünert.   

Abstract

Generally, heat inactivated foetal calf serum (FCS) containing media are used for the cultivation of animal and human cells. The role of serum source and serum treatment on the behaviour of cells has long been neglected. The present study was performed to investigate the effects of serum heat inactivation and serum source on trabecular bone derived progenitor cells (HBC). Furthermore, it was investigated in how far these reactions differed from those seen in bone marrow derived mesenchymal progenitor cells (HBMC) cultures. We found that HBC cultures performed differently in the presence of FCS and HS with or without heat inactivation. The reactions similar to some degree those observed in HBMC cultures. The implications of the results on cell-implant surface interaction studies are discussed.

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Year:  2004        PMID: 15332624     DOI: 10.1023/b:jmsm.0000021127.62879.a1

Source DB:  PubMed          Journal:  J Mater Sci Mater Med        ISSN: 0957-4530            Impact factor:   3.896


  20 in total

1.  Protein adsorption and osteoblast responses to heat-treated titanium surfaces.

Authors:  E Bess; R Cavin; K Ma; J L Ong
Journal:  Implant Dent       Date:  1999       Impact factor: 2.454

2.  The effects of fibroblast growth factor-2 on human neonatal calvaria osteoblastic cells are differentiation stage specific.

Authors:  F Debiais; M Hott; A M Graulet; P J Marie
Journal:  J Bone Miner Res       Date:  1998-04       Impact factor: 6.741

3.  Modulation of osteogenesis and adipogenesis by human serum in human bone marrow cultures.

Authors:  R O Oreffo; A S Virdi; J T Triffitt
Journal:  Eur J Cell Biol       Date:  1997-11       Impact factor: 4.492

4.  Grooves affect primary bone marrow but not osteoblastic MC3T3-E1 cell cultures.

Authors:  A Bruinink; E Wintermantel
Journal:  Biomaterials       Date:  2001-09       Impact factor: 12.479

5.  Human bone cell cultures in biocompatibility testing. Part II: effect of ascorbic acid, beta-glycerophosphate and dexamethasone on osteoblastic differentiation.

Authors:  M J Coelho; M H Fernandes
Journal:  Biomaterials       Date:  2000-06       Impact factor: 12.479

6.  Effects of autologous serum on osteoblastic differentiation in human bone marrow cells.

Authors:  Noriyuki Yamamoto; Masatsugu Isobe; Akihide Negishi; Hidemi Yoshimasu; Hitoyata Shimokawa; Keiichi Ohya; Teruo Amagasa; Shohei Kasugai
Journal:  J Med Dent Sci       Date:  2003-03

7.  Human trabecular bone cells are able to express both osteoblastic and adipocytic phenotype: implications for osteopenic disorders.

Authors:  M E Nuttall; A J Patton; D L Olivera; D P Nadeau; M Gowen
Journal:  J Bone Miner Res       Date:  1998-03       Impact factor: 6.741

8.  Effects of fluoride on human bone cells in vitro: differences in responsiveness between stromal osteoblast precursors and mature osteoblasts.

Authors:  M Kassem; L Mosekilde; E F Eriksen
Journal:  Eur J Endocrinol       Date:  1994-04       Impact factor: 6.664

9.  Characterization of mesenchymal stem cells isolated from murine bone marrow by negative selection.

Authors:  Melody Baddoo; Katy Hill; Robin Wilkinson; Dina Gaupp; Catherine Hughes; Gene C Kopen; Donald G Phinney
Journal:  J Cell Biochem       Date:  2003-08-15       Impact factor: 4.429

10.  Adsorption of fibronectin and vitronectin onto Primaria and tissue culture polystyrene and relationship to the mechanism of initial attachment of human vein endothelial cells and BHK-21 fibroblasts.

Authors:  J G Steele; B A Dalton; G Johnson; P A Underwood
Journal:  Biomaterials       Date:  1995-09       Impact factor: 12.479

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  5 in total

1.  Analysis of human synovial and bone marrow mesenchymal stem cells in relation to heat-inactivation of autologous and fetal bovine serums.

Authors:  Akimoto Nimura; Takeshi Muneta; Koji Otabe; Hideyuki Koga; Young-Jin Ju; Tomoyuki Mochizuki; Koji Suzuki; Ichiro Sekiya
Journal:  BMC Musculoskelet Disord       Date:  2010-09-14       Impact factor: 2.362

2.  Fetal calf serum heat inactivation and lipopolysaccharide contamination influence the human T lymphoblast proteome and phosphoproteome.

Authors:  Hazir Rahman; Muhammad Qasim; Frank C Schultze; Michael Oellerich; Abdul R Asif
Journal:  Proteome Sci       Date:  2011-11-15       Impact factor: 2.480

3.  The Impact of Various Culture Conditions on Human Mesenchymal Stromal Cells Metabolism.

Authors:  Pavla Tonarova; Katerina Lochovska; Robert Pytlik; Marie Hubalek Kalbacova
Journal:  Stem Cells Int       Date:  2021-03-01       Impact factor: 5.443

4.  Alkaline Phosphatase Activity of Serum Affects Osteogenic Differentiation Cultures.

Authors:  Sana Ansari; Keita Ito; Sandra Hofmann
Journal:  ACS Omega       Date:  2022-04-04

Review 5.  The dark side of foetal bovine serum in extracellular vesicle studies.

Authors:  Ornella Urzì; Roger Olofsson Bagge; Rossella Crescitelli
Journal:  J Extracell Vesicles       Date:  2022-10
  5 in total

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