OBJECTIVE: ETS-1 has been identified as a proto-oncogene and a transcription factor for tumor angiogenesis, which is essential for the growth, invasion and metastasis of solid tumors. The aim is to investigate the clinical implications of ETS-1 expression in peritoneal metastatic lesions of ovarian cancers. METHODS: In primary tumors and peritoneal metastatic lesions from 30 patients with stage III ovarian cancers, ETS-1 histoscores and ets-1 mRNA levels were determined by immunohistochemistry and competitive RT-PCR-Southern blot analysis using recombinant RNA, respectively. RESULTS: Immunohistochemical staining revealed that ETS-1 was expressed in the cancer cells and vascular endothelial cells. ETS-1 histoscores in the endothelial cells and ets-1 mRNA levels were significantly (p < 0.05) increased in 20 of 30 peritoneal metastatic lesions of ovarian cancers. There was a significant correlation between microvessel counts (MVCs) and ETS-1 histoscores in the endothelial cells (p < 0.001) and between MVCs and ets-1 mRNA levels in the primary tumor and the peritoneal metastatic lesion of ovarian cancers (p < 0.001). Furthermore, the 24-month survival rate of patients with significantly increased ets-1 mRNA level (2/20, 10%) was significantly (p < 0.01) lower than that of patients with no change in the level (6/10, 60%) from the primary tumor to the peritoneal metastatic lesion. CONCLUSIONS: ETS-1 might be associated with peritoneal metastasis dominantly as an angiogenic mediator and additionally as an oncogene product to activate tumor invasion in ovarian cancers.
OBJECTIVE:ETS-1 has been identified as a proto-oncogene and a transcription factor for tumor angiogenesis, which is essential for the growth, invasion and metastasis of solid tumors. The aim is to investigate the clinical implications of ETS-1 expression in peritoneal metastatic lesions of ovarian cancers. METHODS: In primary tumors and peritoneal metastatic lesions from 30 patients with stage III ovarian cancers, ETS-1 histoscores and ets-1 mRNA levels were determined by immunohistochemistry and competitive RT-PCR-Southern blot analysis using recombinant RNA, respectively. RESULTS: Immunohistochemical staining revealed that ETS-1 was expressed in the cancer cells and vascular endothelial cells. ETS-1 histoscores in the endothelial cells and ets-1 mRNA levels were significantly (p < 0.05) increased in 20 of 30 peritoneal metastatic lesions of ovarian cancers. There was a significant correlation between microvessel counts (MVCs) and ETS-1 histoscores in the endothelial cells (p < 0.001) and between MVCs and ets-1 mRNA levels in the primary tumor and the peritoneal metastatic lesion of ovarian cancers (p < 0.001). Furthermore, the 24-month survival rate of patients with significantly increased ets-1 mRNA level (2/20, 10%) was significantly (p < 0.01) lower than that of patients with no change in the level (6/10, 60%) from the primary tumor to the peritoneal metastatic lesion. CONCLUSIONS:ETS-1 might be associated with peritoneal metastasis dominantly as an angiogenic mediator and additionally as an oncogene product to activate tumor invasion in ovarian cancers.
Authors: Nicolas F Schlecht; Margaret Brandwein-Gensler; Richard V Smith; Nicole Kawachi; Darcy Broughel; Juan Lin; Christian E Keller; Paul A Reynolds; Frank J Gunn-Moore; Thomas Harris; Geoffrey Childs; Thomas J Belbin; Michael B Prystowsky Journal: Head Neck Pathol Date: 2012-01-07
Authors: Jennifer Permuth-Wey; Kate Lawrenson; Howard C Shen; Aneliya Velkova; Jonathan P Tyrer; Zhihua Chen; Hui-Yi Lin; Y Ann Chen; Ya-Yu Tsai; Xiaotao Qu; Susan J Ramus; Rod Karevan; Janet Lee; Nathan Lee; Melissa C Larson; Katja K Aben; Hoda Anton-Culver; Natalia Antonenkova; Antonis C Antoniou; Sebastian M Armasu; François Bacot; Laura Baglietto; Elisa V Bandera; Jill Barnholtz-Sloan; Matthias W Beckmann; Michael J Birrer; Greg Bloom; Natalia Bogdanova; Louise A Brinton; Angela Brooks-Wilson; Robert Brown; Ralf Butzow; Qiuyin Cai; Ian Campbell; Jenny Chang-Claude; Stephen Chanock; Georgia Chenevix-Trench; Jin Q Cheng; Mine S Cicek; Gerhard A Coetzee; Linda S Cook; Fergus J Couch; Daniel W Cramer; Julie M Cunningham; Agnieszka Dansonka-Mieszkowska; Evelyn Despierre; Jennifer A Doherty; Thilo Dörk; Andreas du Bois; Matthias Dürst; Douglas F Easton; Diana Eccles; Robert Edwards; Arif B Ekici; Peter A Fasching; David A Fenstermacher; James M Flanagan; Montserrat Garcia-Closas; Aleksandra Gentry-Maharaj; Graham G Giles; Rosalind M Glasspool; Jesus Gonzalez-Bosquet; Marc T Goodman; Martin Gore; Bohdan Górski; Jacek Gronwald; Per Hall; Mari K Halle; Philipp Harter; Florian Heitz; Peter Hillemanns; Maureen Hoatlin; Claus K Høgdall; Estrid Høgdall; Satoyo Hosono; Anna Jakubowska; Allan Jensen; Heather Jim; Kimberly R Kalli; Beth Y Karlan; Stanley B Kaye; Linda E Kelemen; Lambertus A Kiemeney; Fumitaka Kikkawa; Gottfried E Konecny; Camilla Krakstad; Susanne Krüger Kjaer; Jolanta Kupryjanczyk; Diether Lambrechts; Sandrina Lambrechts; Johnathan M Lancaster; Nhu D Le; Arto Leminen; Douglas A Levine; Dong Liang; Boon Kiong Lim; Jie Lin; Jolanta Lissowska; Karen H Lu; Jan Lubiński; Galina Lurie; Leon F A G Massuger; Keitaro Matsuo; Valerie McGuire; John R McLaughlin; Usha Menon; Francesmary Modugno; Kirsten B Moysich; Toru Nakanishi; Steven A Narod; Lotte Nedergaard; Roberta B Ness; Heli Nevanlinna; Stefan Nickels; Houtan Noushmehr; Kunle Odunsi; Sara H Olson; Irene Orlow; James Paul; Celeste L Pearce; Tanja Pejovic; Liisa M Pelttari; Malcolm C Pike; Elizabeth M Poole; Paola Raska; Stefan P Renner; Harvey A Risch; Lorna Rodriguez-Rodriguez; Mary Anne Rossing; Anja Rudolph; Ingo B Runnebaum; Iwona K Rzepecka; Helga B Salvesen; Ira Schwaab; Gianluca Severi; Viji Shridhar; Xiao-Ou Shu; Yurii B Shvetsov; Weiva Sieh; Honglin Song; Melissa C Southey; Beata Spiewankiewicz; Daniel Stram; Rebecca Sutphen; Soo-Hwang Teo; Kathryn L Terry; Daniel C Tessier; Pamela J Thompson; Shelley S Tworoger; Anne M van Altena; Ignace Vergote; Robert A Vierkant; Daniel Vincent; Allison F Vitonis; Shan Wang-Gohrke; Rachel Palmieri Weber; Nicolas Wentzensen; Alice S Whittemore; Elisabeth Wik; Lynne R Wilkens; Boris Winterhoff; Yin Ling Woo; Anna H Wu; Yong-Bing Xiang; Hannah P Yang; Wei Zheng; Argyrios Ziogas; Famida Zulkifli; Catherine M Phelan; Edwin Iversen; Joellen M Schildkraut; Andrew Berchuck; Brooke L Fridley; Ellen L Goode; Paul D P Pharoah; Alvaro N A Monteiro; Thomas A Sellers; Simon A Gayther Journal: Nat Commun Date: 2013 Impact factor: 14.919