OBJECTIVES: To analyze the contribution of the labile A1C fraction (L-A1C) and sample storage conditions on GHb measurements. DESIGN AND METHODS: L-A1C separation (HPLC Hitachi L-9100) was evaluated before and after sample treatment by four different methods for L-A1C removal. Different sample storage conditions were analyzed. RESULTS: L-A1C may overestimate up to 1.64% total A1C if not properly separated. Samples were stable for up to 10, <1, and at least 100 days for whole blood stored at 4, -20, and -80 degrees C, respectively. CONCLUSIONS: The presence of L-A1C may overestimate GHb results. Samples may be kept under refrigeration up to 10 days and at -80 degrees C if long-term storage is required.
OBJECTIVES: To analyze the contribution of the labile A1C fraction (L-A1C) and sample storage conditions on GHb measurements. DESIGN AND METHODS: L-A1C separation (HPLC Hitachi L-9100) was evaluated before and after sample treatment by four different methods for L-A1C removal. Different sample storage conditions were analyzed. RESULTS: L-A1C may overestimate up to 1.64% total A1C if not properly separated. Samples were stable for up to 10, <1, and at least 100 days for whole blood stored at 4, -20, and -80 degrees C, respectively. CONCLUSIONS: The presence of L-A1C may overestimate GHb results. Samples may be kept under refrigeration up to 10 days and at -80 degrees C if long-term storage is required.
Authors: Marjoriê P Buffon; Mariana P Carpena; Denise A Sortica; Andressa Santer; Rodrigo Carlessi; Bianca M de Souza; Maria I Edelweiss; Milton Berger; Daisy Crispim; Luís H Canani Journal: Diabetol Metab Syndr Date: 2016-01-08 Impact factor: 3.320