OBJECTIVES: Microarray analysis of gene expression profiles of blood leukocytes has many potential clinical and research applications. DESIGN AND METHODS: We used the PAXgene Blood RNA System to prepare RNA from the whole blood of normal volunteers using two incubation times followed by gene expression profiling using the Affymetrix HU133A GeneChip. CONCLUSIONS: Longer incubation gave a significantly higher RNA yield and samples that were satisfactory for microarray analysis, with excellent pairwise correlations between replicates.
OBJECTIVES: Microarray analysis of gene expression profiles of blood leukocytes has many potential clinical and research applications. DESIGN AND METHODS: We used the PAXgene Blood RNA System to prepare RNA from the whole blood of normal volunteers using two incubation times followed by gene expression profiling using the Affymetrix HU133A GeneChip. CONCLUSIONS: Longer incubation gave a significantly higher RNA yield and samples that were satisfactory for microarray analysis, with excellent pairwise correlations between replicates.
Authors: Catherine I Dumur; Maureen Lyons-Weiler; Christin Sciulli; Carleton T Garrett; Iris Schrijver; Tara K Holley; Juan Rodriguez-Paris; Jonathan R Pollack; James L Zehnder; Melissa Price; Jill M Hagenkord; C Ted Rigl; Ljubomir J Buturovic; Glenda G Anderson; Federico A Monzon Journal: J Mol Diagn Date: 2007-12-13 Impact factor: 5.568
Authors: Peter R Sinnaeve; Mark P Donahue; Peter Grass; David Seo; Jacky Vonderscher; Salah-Dine Chibout; William E Kraus; Michael Sketch; Charlotte Nelson; Geoffrey S Ginsburg; Pascal J Goldschmidt-Clermont; Christopher B Granger Journal: PLoS One Date: 2009-09-14 Impact factor: 3.240
Authors: Laura Kennedy; J Keith Vass; D Ross Haggart; Steve Moore; Michael E Burczynski; Dan Crowther; Gino Miele Journal: Biomark Insights Date: 2008-08-25
Authors: Enitan D Carrol; Fiona Salway; Stuart D Pepper; Emma Saunders; Limangeni A Mankhambo; William E Ollier; C Anthony Hart; Phillip Day Journal: BMC Immunol Date: 2007-09-12 Impact factor: 3.615