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Literature DB >> 15327988

Recombinant curculin heterodimer exhibits taste-modifying and sweet-tasting activities.

Maiko Suzuki¹, Eiji Kurimoto, Satoru Nirasawa, Yutaka Masuda, Kouichi Hori, Yoshie Kurihara, Nobuhisa Shimba, Misako Kawai, Ei-Ichiro Suzuki, Koichi Kato.

Abstract

Curculin from Curculigo latifolia is a unique sweet protein that exhibits both sweet-tasting and taste-modifying activities. We isolated a gene that encodes a novel protein highly homologous to curculin. Using cDNAs of the previously known curculin (designated as curculin1) and the novel curculin isoform (curculin2), we produced a panel of homodimeric and heterodimeric recombinant curculins by Escherichia coli expression systems. It was revealed that sweet-tasting and taste-modifying activities were exhibited solely by the heterodimer of curculin1 and curculin2.

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Year: 2004 PMID： 15327988 DOI： 10.1016/j.febslet.2004.07.073

Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124

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Cited

7 in total

1. Structural role of the terminal disulfide bond in the sweetness of brazzein.

Authors: Sannali M Dittli; Hongyu Rao; Marco Tonelli; Jeniffer Quijada; John L Markley; Marianna Max; Fariba Assadi-Porter; Emeline Maillet
Journal: Chem Senses Date: 2011-07-15 Impact factor: 3.160

2. Extracellular production of neoculin, a sweet-tasting heterodimeric protein with taste-modifying activity, by Aspergillus oryzae.

Authors: Ken-ichiro Nakajima; Tomiko Asakura; Jun-ichi Maruyama; Yuji Morita; Hideaki Oike; Akiko Shimizu-Ibuka; Takumi Misaka; Hiroyuki Sorimachi; Soichi Arai; Katsuhiko Kitamoto; Keiko Abe
Journal: Appl Environ Microbiol Date: 2006-05 Impact factor: 4.792

3. Identification and modulation of the key amino acid residue responsible for the pH sensitivity of neoculin, a taste-modifying protein.

Authors: Ken-ichiro Nakajima; Kanako Yokoyama; Taichi Koizumi; Ayako Koizumi; Tomiko Asakura; Tohru Terada; Katsuyoshi Masuda; Keisuke Ito; Akiko Shimizu-Ibuka; Takumi Misaka; Keiko Abe
Journal: PLoS One Date: 2011-04-29 Impact factor: 3.240

4. De novo transcriptome analysis and comparative expression profiling of genes associated with the taste-modifying protein neoculin in Curculigo latifolia and Curculigo capitulata fruits.

Authors: Satoshi Okubo; Kaede Terauchi; Shinji Okada; Yoshikazu Saito; Takao Yamaura; Takumi Misaka; Ken-Ichiro Nakajima; Keiko Abe; Tomiko Asakura
Journal: BMC Genomics Date: 2021-05-13 Impact factor: 3.969

Recombinant curculin heterodimer exhibits taste-modifying and sweet-tasting activities.

1. Structural role of the terminal disulfide bond in the sweetness of brazzein.

2. Extracellular production of neoculin, a sweet-tasting heterodimeric protein with taste-modifying activity, by Aspergillus oryzae.

3. Identification and modulation of the key amino acid residue responsible for the pH sensitivity of neoculin, a taste-modifying protein.

4. De novo transcriptome analysis and comparative expression profiling of genes associated with the taste-modifying protein neoculin in Curculigo latifolia and Curculigo capitulata fruits.

5. Identification of key neoculin residues responsible for the binding and activation of the sweet taste receptor.

6. An efficient in vitro plantlet regeneration from shoot tip cultures of Curculigo latifolia, a medicinal plant.

7. Positive Charges on the Surface of Thaumatin Are Crucial for the Multi-Point Interaction with the Sweet Receptor.