Literature DB >> 1532104

The adenovirus E3-14.5K protein which is required for prevention of TNF cytolysis and for down-regulation of the EGF receptor contains phosphoserine.

P Krajcsi1, W S Wold.   

Abstract

The E3-14.5K and E3-10.4K proteins form a complex and function to down-regulate the epidermal growth factor receptor and to prevent tumor necrosis factor cytolysis in adenovirus-infected cells. Both 14.5K and 10.4K are cytoplasmic membrane proteins with a Ccyt orientation in the membrane. We show here that 14.5K is phosphorylated on serine residues in cells infected by adenoviruses that synthesize both 14.5K and 10.4K. 14.5K is phosphorylated on both serine and threonine in cells infected by a mutant that does not synthesize 10.4K; thus, the presence or absence of 10.4K affects the phosphorylation of 14.5K. Phosphotyrosine was not detected. 14.5K is also phosphorylated when translated in vitro in a rabbit reticulocyte extract. Both in vivo and in vitro, at least one of the phosphorylation sites is near the C-terminus, in the cytoplasmic domain of 14.5K. This C-terminal region of 14.5K is the most conserved among Ad5, Ad2, Ad3, and Ad7, and it is essential for 14.5K to prevent tumor necrosis factor cytolysis.

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Year:  1992        PMID： 1532104     DOI： 10.1016/0042-6822(92)90451-t

Source DB:  PubMed          Journal:  Virology        ISSN： 0042-6822            Impact factor:   3.616


  7 in total

1.  Adenovirus E3-6.7K protein is required in conjunction with the E3-RID protein complex for the internalization and degradation of TRAIL receptor 2.

Authors:  Drew L Lichtenstein; Konstantin Doronin; Karoly Toth; Mohan Kuppuswamy; William S M Wold; Ann E Tollefson
Journal:  J Virol       Date:  2004-11       Impact factor: 5.103

2.  The adenovirus E3-10.4K/14.5K complex mediates loss of cell surface Fas (CD95) and resistance to Fas-induced apoptosis.

Authors:  J Shisler; C Yang; B Walter; C F Ware; L R Gooding
Journal:  J Virol       Date:  1997-11       Impact factor: 5.103

3.  Adenovirus RIDbeta subunit contains a tyrosine residue that is critical for RID-mediated receptor internalization and inhibition of Fas- and TRAIL-induced apoptosis.

Authors:  Drew L Lichtenstein; Peter Krajcsi; David J Esteban; Ann E Tollefson; William S M Wold
Journal:  J Virol       Date:  2002-11       Impact factor: 5.103

4.  Adenovirus E3-10.4K/14.5K protein complex inhibits tumor necrosis factor-induced translocation of cytosolic phospholipase A2 to membranes.

Authors:  T Dimitrov; P Krajcsi; T W Hermiston; A E Tollefson; M Hannink; W S Wold
Journal:  J Virol       Date:  1997-04       Impact factor: 5.103

5.  Distinct domains in the adenovirus E3 RIDalpha protein are required for degradation of Fas and the epidermal growth factor receptor.

Authors:  Tom A Zanardi; Soonpin Yei; Drew L Lichtenstein; Ann E Tollefson; William S M Wold
Journal:  J Virol       Date:  2003-11       Impact factor: 5.103

6.  The adenovirus E3 10.4K and 14.5K proteins, which function to prevent cytolysis by tumor necrosis factor and to down-regulate the epidermal growth factor receptor, are localized in the plasma membrane.

Authors:  A R Stewart; A E Tollefson; P Krajcsi; S P Yei; W S Wold
Journal:  J Virol       Date:  1995-01       Impact factor: 5.103

Review 7.  Immunomodulatory functions encoded by the E3 transcription unit of adenoviruses.

Authors:  H G Burgert; J H Blusch
Journal:  Virus Genes       Date:  2000       Impact factor: 2.198
  7 in total

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