BACKGROUND/AIMS: We sought to characterize a novel adsorbent polymer in terms of cytokine removal. METHODS: We challenged 50 rats with lipopolysaccharide to obtain cytokine-rich blood and circulated this through cartridges containing polymer. In separate experiments, cell-free supernatants were passed through cartridges containing polymer. We measured tumor necrosis factor alpha, interleukin 10 and interleukin 6 concentrations under a variety of conditions to evaluate adsorption kinetics. RESULTS: All three cytokines were rapidly removed from the blood with less than 50% of the initial concentrations present after 1 h of circulation through the cartridge. There was no significant difference in the effect across a range of blood flows and Ca2+ concentrations. Adsorption was decreased somewhat by extremely low temperature (4 degrees C). CONCLUSION: The adsorbent polymer removes cytokines with high efficiency, and binding is relatively unaffected by a variety of physical conditions.
BACKGROUND/AIMS: We sought to characterize a novel adsorbent polymer in terms of cytokine removal. METHODS: We challenged 50 rats with lipopolysaccharide to obtain cytokine-rich blood and circulated this through cartridges containing polymer. In separate experiments, cell-free supernatants were passed through cartridges containing polymer. We measured tumor necrosis factor alpha, interleukin 10 and interleukin 6 concentrations under a variety of conditions to evaluate adsorption kinetics. RESULTS: All three cytokines were rapidly removed from the blood with less than 50% of the initial concentrations present after 1 h of circulation through the cartridge. There was no significant difference in the effect across a range of blood flows and Ca2+ concentrations. Adsorption was decreased somewhat by extremely low temperature (4 degrees C). CONCLUSION: The adsorbent polymer removes cytokines with high efficiency, and binding is relatively unaffected by a variety of physical conditions.
Authors: N J Lees; Ajp Rosenberg; A I Hurtado-Doce; J Jones; N Marczin; M Zeriouh; A Weymann; A Sabashnikov; A R Simon; A F Popov Journal: J Artif Organs Date: 2016-07-19 Impact factor: 1.731
Authors: Jeremy D Kimmel; Gregory A Gibson; Simon C Watkins; John A Kellum; William J Federspiel Journal: J Biomed Mater Res B Appl Biomater Date: 2010-02 Impact factor: 3.368
Authors: Krasimira M Mikhova; Creighton W Don; Michael Laflamme; John A Kellum; Michael S Mulligan; Edward D Verrier; David G Rabkin Journal: J Thorac Cardiovasc Surg Date: 2012-11-03 Impact factor: 5.209