OBJECTIVE: The purpose of this study was to investigate the morphological, histochemical, and immunocytochemical changes of the oral mucosa after CO(2) or Er:YAG laser irradiation. BACKGROUND DATA: There have been no comparative reports on CO(2) and Er:YAG laser effects on human oral soft tissues. MATERIALS AND METHODS:Tissue preservation was studied in 40 oral biopsies of young patients obtained by CO(2) and Er:YAG laser surgery. Hematoxylin-eosin and Giemsa stains, PAS/diastase treatment, AE1 and AE2 cytokeratins, MiB1/Ki67, and bcl-2 immunoreactions were performed on the laser cut edges on formalin fixed, paraffin embedded biopsies. RESULTS:CO(2) laser biopsies show blisters, clefts and erosions of the epithelium. Intracellular edema and lengthened nuclei were also seen. The glycogen content results decreased in CO(2) laser biopsies. Good expression for cytokeratins and cell-cycle proliferation markers were found in Er:YAG biopsies, on the contrary the apoptosis marker was better expressed in CO(2) laser biopsies. CONCLUSION: The results suggest that Er:YAG laser may be routinely used in surgery, because of its minimal damage of the epithelial tissue, its low inflammatory reaction, its quicker healing process and its lower risk of scarring.
RCT Entities:
OBJECTIVE: The purpose of this study was to investigate the morphological, histochemical, and immunocytochemical changes of the oral mucosa after CO(2) or Er:YAG laser irradiation. BACKGROUND DATA: There have been no comparative reports on CO(2) and Er:YAG laser effects on human oral soft tissues. MATERIALS AND METHODS: Tissue preservation was studied in 40 oral biopsies of young patients obtained by CO(2) and Er:YAG laser surgery. Hematoxylin-eosin and Giemsa stains, PAS/diastase treatment, AE1 and AE2 cytokeratins, MiB1/Ki67, and bcl-2 immunoreactions were performed on the laser cut edges on formalin fixed, paraffin embedded biopsies. RESULTS:CO(2) laser biopsies show blisters, clefts and erosions of the epithelium. Intracellular edema and lengthened nuclei were also seen. The glycogen content results decreased in CO(2) laser biopsies. Good expression for cytokeratins and cell-cycle proliferation markers were found in Er:YAG biopsies, on the contrary the apoptosis marker was better expressed in CO(2) laser biopsies. CONCLUSION: The results suggest that Er:YAG laser may be routinely used in surgery, because of its minimal damage of the epithelial tissue, its low inflammatory reaction, its quicker healing process and its lower risk of scarring.
Authors: Felipe Fornias Sperandio; Daiane T Meneguzzo; Leila S Ferreira; Patrícia A da Ana; Luciane H Azevedo; Suzana C O M de Sousa Journal: Lasers Med Sci Date: 2010-11-03 Impact factor: 3.161
Authors: Markus Hess; Michael Dominik Hildebrandt; Frank Müller; Sebastian Kruber; Peter Kroetz; Udo Schumacher; Rudolph Reimer; Michael Kammal; Klaus Püschel; Wolfgang Wöllmer; Dwayne Miller Journal: Eur Arch Otorhinolaryngol Date: 2013-05-25 Impact factor: 2.503
Authors: Marina Consuelo Vitale; Maria Gloria Nardi; Matteo Pellegrini; Francesco Spadari; Federica Pulicari; Roberto Alcozer; Martina Minardi; Maria Francesca Sfondrini; Karin Bertino; Andrea Scribante Journal: Case Rep Dent Date: 2022-10-06