Literature DB >> 15315352

Expression of transforming growth factor-beta in cultured normal human lens epithelia cells.

Yukan Huang1, Houren Wei.   

Abstract

In order to investigate whether cultured normal human lens epithelial cells (LEC) express transforming growth factor beta (TGF-beta), reverse transcriptase polymerase chain reaction (RT-PCR) and immunohistochemical methods were used for detection of TGF-beta mRNA and protein in cultured normal human LEC. The results showed that a single RT-PCR amplified product about 310bp was obtained, and the sequence was homologous to the known sequence. TGF-beta immunostain was positive in the plasma of LEC. It was suggested that normal human LEC could produce TGF-beta, and LEC could be affected by TGF-beta through autocrine action.

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Year:  2004        PMID： 15315352     DOI： 10.1007/bf02832016

Source DB:  PubMed          Journal:  J Huazhong Univ Sci Technolog Med Sci        ISSN： 1672-0733


  11 in total

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Journal:  J Exp Med       Date:  1997-01-20       Impact factor: 14.307

5.  Expression of transforming growth factor (TGF)-alpha, TGF-beta(2) and interleukin 8 messenger RNA in postsurgical and cultured lens epithelial cells obtained from patients with senile cataracts.

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Journal:  Graefes Arch Clin Exp Ophthalmol       Date:  1999-10       Impact factor: 3.117

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Journal:  Invest Ophthalmol Vis Sci       Date:  1999-08       Impact factor: 4.799

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Journal:  J Clin Invest       Date:  1998-02-01       Impact factor: 14.808

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Journal:  Trans Ophthalmol Soc U K       Date:  1982

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Authors:  A M Hales; M W Schulz; C G Chamberlain; J W McAvoy
Journal:  Curr Eye Res       Date:  1994-12       Impact factor: 2.424

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Authors:  A M Hales; C G Chamberlain; J W McAvoy
Journal:  Invest Ophthalmol Vis Sci       Date:  1995-07       Impact factor: 4.799
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  1 in total

1.  Expression of TGF-beta2 mRNA and PCNA, FN protein in lens epithelial cells in age-related nuclear and cortex cataract.

Authors:  Lin Ye; Qin Li; Xiaojun Cai; Ping Deng
Journal:  J Huazhong Univ Sci Technolog Med Sci       Date:  2005
  1 in total

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