Literature DB >> 15315325

Preparation and characterization of polyclonal antibodies against VLDL receptor.

Shen Qu1, Tao Chen, Fan Wu, Yanhua Yin, Hao Bi.   

Abstract

The polyclonal antibodies against VLDL receptor were prepared and identified. Rabbits were immunized with polypeptide fragment of VLDL receptor as antigen. The collected blood serum of the immunized rabbits was analyzed and identified by using ELISA and Western Blot. The results showed that the rabbit against mouse and human VLDL receptor antibodies were obtained with high titer and could recognize the natural VLDL receptors through Western blot. The prepared polyclonal antibodies against VLDL receptor provide a new tool to study the protein of VLDL receptor.

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Year:  2004        PMID： 15315325     DOI： 10.1007/bf02831989

Source DB:  PubMed          Journal:  J Huazhong Univ Sci Technolog Med Sci        ISSN： 1672-0733


  5 in total

1.  Very low-density lipoprotein receptor in fetal intestine and gastric adenocarcinoma cells.

Authors:  Y Nakamura; M Yamamoto; E Kumamaru
Journal:  Arch Pathol Lab Med       Date:  2000-01       Impact factor: 5.534

2.  Ligand binding properties of the very low density lipoprotein receptor. Absence of the third complement-type repeat encoded by exon 4 is associated with reduced binding of Mr 40,000 receptor-associated protein.

Authors:  P M Rettenberger; K Oka; L Ellgaard; H H Petersen; A Christensen; P M Martensen; D Monard; M Etzerodt; L Chan; P A Andreasen
Journal:  J Biol Chem       Date:  1999-03-26       Impact factor: 5.157

3.  Mouse very low-density lipoprotein receptor (VLDLR): gene structure, tissue-specific expression and dietary and developmental regulation.

Authors:  O Tiebel; K Oka; K Robinson; M Sullivan; J Martinez; M Nakamuta; K Ishimura-Oka; L Chan
Journal:  Atherosclerosis       Date:  1999-08       Impact factor: 5.162

4.  Expression of LDL receptor, VLDL receptor, LDL receptor-related protein, and scavenger receptor in rabbit atherosclerotic lesions: marked induction of scavenger receptor and VLDL receptor expression during lesion development.

Authors:  T P Hiltunen; J S Luoma; T Nikkari; S Ylä-Herttuala
Journal:  Circulation       Date:  1998-03-24       Impact factor: 29.690

5.  Expression and characterization of a very low density lipoprotein receptor variant lacking the O-linked sugar region generated by alternative splicing.

Authors:  H Iijima; M Miyazawa; J Sakai; K Magoori; M R Ito; H Suzuki; M Nose; Y Kawarabayasi; T T Yamamoto
Journal:  J Biochem       Date:  1998-10       Impact factor: 3.387
  5 in total
  1 in total

1.  Variations of very low-density lipoprotein receptor subtype expression in gastrointestinal adenocarcinoma cells with various differentiations.

Authors:  Tao Chen; Fan Wu; Feng-Ming Chen; Jun Tian; Shen Qu
Journal:  World J Gastroenterol       Date:  2005-05-14       Impact factor: 5.742
  1 in total

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