Hui Zhang1, Shao-hua Zhu, Qi-sheng Qin. 1. Department of Forensic Pathology, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China. zhanghui7879@hotmail.com
Abstract
OBJECTIVE: To explore the expression of interleukin-10 (IL-10) in different expressive parts and its relationship with the wound age during cutaneous wound healing in mice. METHODS: Immunohistochemical and morphometrical analysis methods were performed on vital skin wounds. RESULTS: In normal and injury mouse skin IL-10 immunoreactivity was observed in epidermal cells. The expression of IL-10 in epidermal cells was already enhanced since 1-3h after incision, and it returned to lower lever by 24h, but increased again at 48h after injury. IL-10 immunoreactivive cells under epidermis were mainly infiltrating mononuclear cells. Morphometrically, the ratio of the number of infiltrating cells was evaluated. A subsequent increase in the IL-10 positive cells was observed in the granulation tissue beyond the wound and in dermal at 6h after incision, and the maximum ratio was (51.41 +/- 3.12)% in a 72h-old wound. Then the positive cells gradually decreased (29.38 +/- 2.64)%-(5.56 +/- 4.74)% after incision 96-168h. CONCLUSIONS: The expression characteristics of IL-10 in different expressive parts should be related to the wound age and morphometry of different expressive parts may be employed for determining the wound age.
OBJECTIVE: To explore the expression of interleukin-10 (IL-10) in different expressive parts and its relationship with the wound age during cutaneous wound healing in mice. METHODS: Immunohistochemical and morphometrical analysis methods were performed on vital skin wounds. RESULTS: In normal and injury mouse skin IL-10 immunoreactivity was observed in epidermal cells. The expression of IL-10 in epidermal cells was already enhanced since 1-3h after incision, and it returned to lower lever by 24h, but increased again at 48h after injury. IL-10 immunoreactivive cells under epidermis were mainly infiltrating mononuclear cells. Morphometrically, the ratio of the number of infiltrating cells was evaluated. A subsequent increase in the IL-10 positive cells was observed in the granulation tissue beyond the wound and in dermal at 6h after incision, and the maximum ratio was (51.41 +/- 3.12)% in a 72h-old wound. Then the positive cells gradually decreased (29.38 +/- 2.64)%-(5.56 +/- 4.74)% after incision 96-168h. CONCLUSIONS: The expression characteristics of IL-10 in different expressive parts should be related to the wound age and morphometry of different expressive parts may be employed for determining the wound age.