Literature DB >> 15310923

Histochemical and fluorometric assays for uidA (GUS) gene detection.

Magdalena Cervera1.   

Abstract

Transgenic plant production has been intimately connected to the beta-glucuronidase (uidA or GUS) gene used as a reporter marker gene. The enzyme stability and the high sensitivity and amenability of the GUS assay to qualitative (histochemical assay) and to quantitative (fluorometric or spectrophotometric assay) detection are some of the reasons that explain the extensive use of uidA gene in plant genetic transformation. Methods for uidA gene detection have been thoroughly described in the literature. The aim of this chapter is to describe the basic protocols needed for GUS detection in a plant genetic transformation laboratory.

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Year:  2005        PMID： 15310923     DOI： 10.1385/1-59259-827-7:203

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  24 in total

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Journal:  Appl Environ Microbiol       Date:  2010-07-02       Impact factor: 4.792

2.  Evaluation of selection strategies alternative to nptII in genetic transformation of citrus.

Authors:  Alida Ballester; Magdalena Cervera; Leandro Peña
Journal:  Plant Cell Rep       Date:  2008-03-04       Impact factor: 4.570

3.  Development and optimization of agroinfiltration for soybean.

Authors:  Jessica L King; John J Finer; Leah K McHale
Journal:  Plant Cell Rep       Date:  2014-10-19       Impact factor: 4.570

4.  A red fluorescent protein (DsRED) from Discosoma sp. as a reporter for gene expression in walnut somatic embryos.

Authors:  Qixiang Zhang; Sriema L Walawage; David M Tricoli; Abhaya M Dandekar; Charles A Leslie
Journal:  Plant Cell Rep       Date:  2015-01-28       Impact factor: 4.570

5.  A petunia ABC protein controls strigolactone-dependent symbiotic signalling and branching.

Authors:  Tobias Kretzschmar; Wouter Kohlen; Joelle Sasse; Lorenzo Borghi; Markus Schlegel; Julien B Bachelier; Didier Reinhardt; Ralph Bours; Harro J Bouwmeester; Enrico Martinoia
Journal:  Nature       Date:  2012-03-07       Impact factor: 49.962

6.  Deficiency in riboflavin biosynthesis affects tetrapyrrole biosynthesis in etiolated Arabidopsis tissue.

Authors:  Boris Hedtke; Ali Alawady; Alfonso Albacete; Koichi Kobayashi; Michael Melzer; Thomas Roitsch; Tatsuru Masuda; Bernhard Grimm
Journal:  Plant Mol Biol       Date:  2011-11-13       Impact factor: 4.076

7.  Efficient production of transgenic citrus plants using isopentenyl transferase positive selection and removal of the marker gene by site-specific recombination.

Authors:  Alida Ballester; Magdalena Cervera; Leandro Peña
Journal:  Plant Cell Rep       Date:  2006-08-23       Impact factor: 4.570

8.  Differential resistance to Citrus psorosis virus in transgenic Nicotiana benthamiana plants expressing hairpin RNA derived from the coat protein and 54K protein genes.

Authors:  Carina Andrea Reyes; Eduardo José Peña; María Cecilia Zanek; Daniela Verónica Sanchez; Oscar Grau; María Laura García
Journal:  Plant Cell Rep       Date:  2009-10-10       Impact factor: 4.570

9.  Separating the roles of acropetal and basipetal auxin transport on gravitropism with mutations in two Arabidopsis multidrug resistance-like ABC transporter genes.

Authors:  Daniel R Lewis; Nathan D Miller; Bessie L Splitt; Guosheng Wu; Edgar P Spalding
Journal:  Plant Cell       Date:  2007-06-08       Impact factor: 11.277

10.  Impact of ubiquitous inhibitors on the GUS gene reporter system: evidence from the model plants Arabidopsis, tobacco and rice and correction methods for quantitative assays of transgenic and endogenous GUS.

Authors:  Simone Fior; Paolo D Gerola
Journal:  Plant Methods       Date:  2009-12-30       Impact factor: 4.993
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