| Literature DB >> 15310279 |
A V Ivanov1, M V Kozlov, A O Kuzyakin, D A Kostyuk, V L Tunitskaya, S N Kochetkov.
Abstract
Recombinant RNA-dependent RNA polymerase of hepatitis C virus was purified using a bacterial expression system (Escherichia coli). The system for enzyme activity detection was optimized. The maximum activity was achieved when the reaction was carried out at 30 degrees C in the presence of 3 mM Mg2+ or 0.75 mM Mn2+. Among alpha- and beta-pyrogallaldehydes, effective inhibitors were found. It was shown that they acted at the primer elongation stage, and their binding to the protein is reversible.Entities:
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Year: 2004 PMID： 15310279 DOI： 10.1023/b:biry.0000040204.82885.f1
Source DB: PubMed Journal: Biochemistry (Mosc) ISSN： 0006-2979 Impact factor: 2.487