BACKGROUND: It is now generally accepted that coeliac disease (CD) is caused by inflammatory T cell responses to gluten peptides bound to HLA-DQ2 or -DQ8 molecules. There is overwhelming evidence that CD patients can mount T cell responses to peptides found in both alpha-gliadin and gamma-gliadin molecules. Assays that would detect the presence or absence of such peptides in food would thus be accurate indicators of safety for consumption by CD patients. AIMS: The development of a sensitive method to detect T cell stimulatory epitopes of alpha-gliadin and gamma-gliadin molecules in food products. METHODS: Monoclonal antibodies (mAb) were raised against peptides encoding the T cell stimulatory epitopes of alpha-gliadin (amino acids (aa) 59-71) and aa gamma-gliadin (aa 142-153 and aa 147-159). These mAb competition assays were developed that quantitatively detect T cell stimulatory epitopes present on both intact proteins and peptides of sizes recognisable by CD4(+) T cells. RESULTS: With the mAb based competition assays, T cell epitopes were detected in pepsin/trypsin digests of wheat proteins and ethanol extracts of various food products, with detection levels lower than those reached with gluten specific T cells. Moreover, the presence of T cell stimulatory epitopes was also detected in preparations of barley, rye, and triticale, other cereals known to be toxic for CD patients. CONCLUSIONS: A new antibody based method has been developed, detecting the presence of T cell stimulatory gluten peptides. This can be used to further ensure the safety of food consumed by CD patients.
BACKGROUND: It is now generally accepted that coeliac disease (CD) is caused by inflammatory T cell responses to gluten peptides bound to HLA-DQ2 or -DQ8 molecules. There is overwhelming evidence that CDpatients can mount T cell responses to peptides found in both alpha-gliadin and gamma-gliadin molecules. Assays that would detect the presence or absence of such peptides in food would thus be accurate indicators of safety for consumption by CDpatients. AIMS: The development of a sensitive method to detect T cell stimulatory epitopes of alpha-gliadin and gamma-gliadin molecules in food products. METHODS: Monoclonal antibodies (mAb) were raised against peptides encoding the T cell stimulatory epitopes of alpha-gliadin (amino acids (aa) 59-71) and aa gamma-gliadin (aa 142-153 and aa 147-159). These mAb competition assays were developed that quantitatively detect T cell stimulatory epitopes present on both intact proteins and peptides of sizes recognisable by CD4(+) T cells. RESULTS: With the mAb based competition assays, T cell epitopes were detected in pepsin/trypsin digests of wheat proteins and ethanol extracts of various food products, with detection levels lower than those reached with gluten specific T cells. Moreover, the presence of T cell stimulatory epitopes was also detected in preparations of barley, rye, and triticale, other cereals known to be toxic for CDpatients. CONCLUSIONS: A new antibody based method has been developed, detecting the presence of T cell stimulatory gluten peptides. This can be used to further ensure the safety of food consumed by CDpatients.
Authors: E K Janatuinen; T A Kemppainen; R J K Julkunen; V-M Kosma; M Mäki; M Heikkinen; M I J Uusitupa Journal: Gut Date: 2002-03 Impact factor: 23.059
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Authors: Hetty C van den Broeck; Hein C de Jong; Elma M J Salentijn; Liesbeth Dekking; Dirk Bosch; Rob J Hamer; Ludovicus J W J Gilissen; Ingrid M van der Meer; Marinus J M Smulders Journal: Theor Appl Genet Date: 2010-07-28 Impact factor: 5.699
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Authors: B N Salden; V Monserrat; F J Troost; M J Bruins; L Edens; R Bartholomé; G R Haenen; B Winkens; F Koning; A A Masclee Journal: Aliment Pharmacol Ther Date: 2015-06-04 Impact factor: 8.171
Authors: Hetty C van den Broeck; Teun W J M van Herpen; Cees Schuit; Elma M J Salentijn; Liesbeth Dekking; Dirk Bosch; Rob J Hamer; Marinus J M Smulders; Ludovicus J W J Gilissen; Ingrid M van der Meer Journal: BMC Plant Biol Date: 2009-04-07 Impact factor: 4.215
Authors: Elma Mj Salentijn; Svetlana V Goryunova; Noor Bas; Ingrid M van der Meer; Hetty C van den Broeck; Thomas Bastien; Luud J W J Gilissen; Marinus J M Smulders Journal: BMC Genomics Date: 2009-01-26 Impact factor: 3.969