| Literature DB >> 15302164 |
Herbert Bosshart1, Michael Heinzelmann.
Abstract
The involvement of cytosolic free calcium (Ca2+) as a second messenger in lipopolysaccharide (LPS)-activated cellular pathways remains controversial. Using human monocyte-like THP-1 cells stably transfected with glycosylphosphatidylinositol-anchored human CD14, we have monitored the concentration of intracellular Ca2+ after sequential addition of LPS, ATP, and ionomycin. Whereas ATP and ionomycin induced rapid elevations of cytosolic Ca2+, no such changes were observed after LPS addition. These data argue against the participation of cytosolic Ca2+ in the CD14-dependent LPS activation pathway.Entities:
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Year: 2004 PMID： 15302164 DOI： 10.1016/j.molimm.2004.05.003
Source DB: PubMed Journal: Mol Immunol ISSN： 0161-5890 Impact factor: 4.407