AIM: To construct a recombinant strain which expresses BabA of Helicobacter pylori (H pylori) and to study the immunogenicity of BabA. METHODS: BabA2 DNA was amplified by PCR and inserted into the prokaryotie expression vector pET-22b (+) and expressed in the BL21 (DE3) E.coli strain. Furthermore, BabA immunogenicity was studied by animal test. RESULTS: DNA sequence analysis showed the sequence of BabA2 DNA was the same as the one published by GenBank. The BabA recombinant protein accounted for 34.8% of the total bacterial protein. The serum from H pylori infected patients and Balb/c miced immunized with BabA itself could recognize rBabA. CONCLUSION: BabA recombinant protein may be an potential vaccine for control and treatment of H pylori infection. Copyright 2004 The WJG Press ISSN
AIM: To construct a recombinant strain which expresses BabA of Helicobacter pylori (H pylori) and to study the immunogenicity of BabA. METHODS: BabA2 DNA was amplified by PCR and inserted into the prokaryotie expression vector pET-22b (+) and expressed in the BL21 (DE3) E.coli strain. Furthermore, BabA immunogenicity was studied by animal test. RESULTS: DNA sequence analysis showed the sequence of BabA2 DNA was the same as the one published by GenBank. The BabA recombinant protein accounted for 34.8% of the total bacterial protein. The serum from H pylori infectedpatients and Balb/c miced immunized with BabA itself could recognize rBabA. CONCLUSION: BabA recombinant protein may be an potential vaccine for control and treatment of H pylori infection. Copyright 2004 The WJG Press ISSN
Authors: J A Bosch; E J de Geus; T J Ligtenberg; K Nazmi; E C Veerman; J Hoogstraten; A V Amerongen Journal: Psychosom Med Date: 2000 Jan-Feb Impact factor: 4.312
Authors: A Morgner; S Miehlke; M Stolte; A Neubauer; B Alpen; C Thiede; H Klann; F X Hierlmeier; C Ell; G Ehninger; E Bayerdörffer Journal: World J Gastroenterol Date: 2001-04 Impact factor: 5.742
Authors: D Ilver; A Arnqvist; J Ogren; I M Frick; D Kersulyte; E T Incecik; D E Berg; A Covacci; L Engstrand; T Borén Journal: Science Date: 1998-01-16 Impact factor: 47.728
Authors: A Covacci; S Censini; M Bugnoli; R Petracca; D Burroni; G Macchia; A Massone; E Papini; Z Xiang; N Figura Journal: Proc Natl Acad Sci U S A Date: 1993-06-15 Impact factor: 11.205