Literature DB >> 15299491

Structure of the crystalline complex of cytidylic acid (2'-CMP) with ribonuclease at 1.6 A resolution. Conservation of solvent sites in RNase-A high-resolution structures.

J N Lisgarten1, V Gupta, D Maes, L Wyns, I Zegers, R A Palmer, C G Dealwis, C F Aguilar, A M Hemmings.   

Abstract

The X-ray structure of the inhibitor complex of bovine ribonuclease A with cytidylic acid (2'-CMP) has been determined at 1.6 A resolution and refined by restrained least squares to R = 0.17 for 11 945 reflections. Binding of the inhibitor molecule to the protein is confirmed to be in the productive mode associated with enzyme activity. A study of conserved solvent sites amongst high-resolution structures in the same crystal form reveals a stabilizing water cluster between the N and C termini.

Entities:  

Year:  1993        PMID： 15299491     DOI： 10.1107/S090744499300719X

Source DB:  PubMed          Journal:  Acta Crystallogr D Biol Crystallogr        ISSN： 0907-4449


  7 in total

1.  Ternary borate-nucleoside complex stabilization by ribonuclease A demonstrates phosphate mimicry.

Authors:  Scott A Gabel; Robert E London
Journal:  J Biol Inorg Chem       Date:  2007-10-24       Impact factor: 3.358

2.  Productive and nonproductive binding to ribonuclease A: X-ray structure of two complexes with uridylyl(2',5')guanosine.

Authors:  L Vitagliano; A Merlino; A Zagari; L Mazzarella
Journal:  Protein Sci       Date:  2000-06       Impact factor: 6.725

3.  Insights to the Structural Basis for the Stereospecificity of the Escherichia coli Phytase, AppA.

Authors:  Isabella M Acquistapace; Emma J Thompson; Imke Kühn; Mike R Bedford; Charles A Brearley; Andrew M Hemmings
Journal:  Int J Mol Sci       Date:  2022-06-06       Impact factor: 6.208

4.  The structures of RNase A complexed with 3'-CMP and d(CpA): active site conformation and conserved water molecules.

Authors:  I Zegers; D Maes; M H Dao-Thi; F Poortmans; R Palmer; L Wyns
Journal:  Protein Sci       Date:  1994-12       Impact factor: 6.725

5.  Three-dimensional structure of the complexes of ribonuclease A with 2',5'-CpA and 3',5'-d(CpA) in aqueous solution, as obtained by NMR and restrained molecular dynamics.

Authors:  C Toiron; C González; M Bruix; M Rico
Journal:  Protein Sci       Date:  1996-08       Impact factor: 6.725

6.  High-resolution crystal structures of ribonuclease A complexed with adenylic and uridylic nucleotide inhibitors. Implications for structure-based design of ribonucleolytic inhibitors.

Authors:  Demetres D Leonidas; Gayatri B Chavali; Nikos G Oikonomakos; Evangelia D Chrysina; Magda N Kosmopoulou; Metaxia Vlassi; Claire Frankling; K Ravi Acharya
Journal:  Protein Sci       Date:  2003-11       Impact factor: 6.725

7.  Representation of protein 3D structures in spherical (ρ, ϕ, θ) coordinates and two of its potential applications.

Authors:  Vicente M Reyes
Journal:  Interdiscip Sci       Date:  2011-09-29       Impact factor: 2.233
  7 in total

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