Literature DB >> 15299399

Crystallization experiments with 2-enoyl-CoA hydratase, using an automated 'fast-screening' crystallization protocol.

J P Zeelen1, J K Hiltunen, T A Ceska, R K Wierenga.   

Abstract

A convenient method for screening crystallization conditions using an automated fast-screen protocol has been implemented and tested on an enoyl-CoA hydratase. The crystallization solutions for the initial screening and subsequent optimizations are prepared using a crystallization robot. Enoyl-CoA hydratase (E.C. 4.2.1.17), purified from rat-liver mitochondria, is one of the enzymes from the beta-oxidation pathway of fatty-acid metabolism; it catalyzes the reversible hydration of 2-trans-enoyl-CoA's to L-3-hydroxy-acyl-CoA's. Different crystal forms, diffracting to 3.0 A, were obtained.

Entities:  

Year:  1994        PMID： 15299399     DOI： 10.1107/S0907444994001277

Source DB:  PubMed          Journal:  Acta Crystallogr D Biol Crystallogr        ISSN： 0907-4449


  8 in total

1.  Crystal structures of nitroalkane oxidase: insights into the reaction mechanism from a covalent complex of the flavoenzyme trapped during turnover.

Authors:  Akanksha Nagpal; Michael P Valley; Paul F Fitzpatrick; Allen M Orville
Journal:  Biochemistry       Date:  2006-01-31       Impact factor: 3.162

2.  Structural studies of Streptococcus pyogenes streptolysin O provide insights into the early steps of membrane penetration.

Authors:  Susanne C Feil; David B Ascher; Michael J Kuiper; Rodney K Tweten; Michael W Parker
Journal:  J Mol Biol       Date:  2013-12-03       Impact factor: 5.469

3.  Nonactin biosynthesis: the product of nonS catalyzes the formation of the furan ring of nonactic acid.

Authors:  A J Woo; W R Strohl; N D Priestley
Journal:  Antimicrob Agents Chemother       Date:  1999-07       Impact factor: 5.191

4.  Crystallization and preliminary crystallographic studies of the copper-binding domain of the amyloid precursor protein of Alzheimer's disease.

Authors:  Geoffrey K-W Kong; Denise Galatis; Kevin J Barnham; Galina Polekhina; Julian J Adams; Colin L Masters; Roberto Cappai; Michael W Parker; William J McKinstry
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2004-12-02

5.  Crystal structure of enoyl-coenzyme A (CoA) hydratase at 2.5 angstroms resolution: a spiral fold defines the CoA-binding pocket.

Authors:  C K Engel; M Mathieu; J P Zeelen; J K Hiltunen; R K Wierenga
Journal:  EMBO J       Date:  1996-10-01       Impact factor: 11.598

6.  Structure of the lectin regulatory domain of the cholesterol-dependent cytolysin lectinolysin reveals the basis for its lewis antigen specificity.

Authors:  Susanne C Feil; Sara Lawrence; Terrence D Mulhern; Jessica K Holien; Eileen M Hotze; Stephen Farrand; Rodney K Tweten; Michael W Parker
Journal:  Structure       Date:  2012-02-08       Impact factor: 5.006

7.  Structural basis for the catalytic mechanism and α-ketoglutarate cooperativity of glutamate dehydrogenase.

Authors:  Prem Prakash; Narayan S Punekar; Prasenjit Bhaumik
Journal:  J Biol Chem       Date:  2018-03-14       Impact factor: 5.157

8.  Crystal structure of human anterior gradient protein 3.

Authors:  Van Dat Nguyen; Ekaterina Biterova; Mikko Salin; Rik K Wierenga; Lloyd W Ruddock
Journal:  Acta Crystallogr F Struct Biol Commun       Date:  2018-06-26       Impact factor: 1.056
  8 in total

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