Literature DB >> 15297555

Evaluation of a new automated, standardized generic nucleic acid extraction method (total nucleic acid isolation kit) used in combination with cytomegalovirus DNA quantification by COBAS AMPLICOR CMV MONITOR.

Barbara Christine Gärtner1, Johannes Michael Fischinger, Anke Litwicki, Klaus Roemer, Nikolaus Mueller-Lantzsch.   

Abstract

The new generic Total Nucleic Acid Isolation kit improved the detection limit of a cytomegalovirus (CMV) PCR from 400 to 200 copies/ml. Sensitivity and specificity in 30 CMV-positive and 100 negative samples were 100%. Analytical performance was excellent. The kit provides a reliable, standardized, and time-saving tool for DNA extraction.

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Year:  2004        PMID： 15297555      PMCID： PMC497653          DOI： 10.1128/JCM.42.8.3881-3882.2004

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  7 in total

1.  High variability between results of different in-house tests for cytomegalovirus (CMV) monitoring and a standardized quantitative plasma CMV PCR assay.

Authors:  Lutz Von Müller; Walter Hampl; Joachim Hinz; Helga Meisel; Angela Reip; Elisabeth Engelmann; Regine Heilbronn; Barbara Gärtner; Oliver Krämer; Hermann Einsele; Holger Hebart; Tatjana Ljubicic; Jürgen Löffler; Thomas Mertens
Journal:  J Clin Microbiol       Date:  2002-06       Impact factor: 5.948

2.  Evaluation of the COBAS AMPLICOR CMV MONITOR test for detection of viral DNA in specimens taken from patients after liver transplantation.

Authors:  I G Sia; J A Wilson; M J Espy; C V Paya; T F Smith
Journal:  J Clin Microbiol       Date:  2000-02       Impact factor: 5.948

3.  Rapid and simple method for purification of nucleic acids.

Authors:  R Boom; C J Sol; M M Salimans; C L Jansen; P M Wertheim-van Dillen; J van der Noordaa
Journal:  J Clin Microbiol       Date:  1990-03       Impact factor: 5.948

4.  COBAS AMPLICOR: fully automated RNA and DNA amplification and detection system for routine diagnostic PCR.

Authors:  N DiDomenico; H Link; R Knobel; T Caratsch; W Weschler; Z G Loewy; M Rosenstraus
Journal:  Clin Chem       Date:  1996-12       Impact factor: 8.327

5.  Evaluation of the AMPLICOR CMV test for direct detection of cytomegalovirus in plasma specimens.

Authors:  M Hiyoshi; S Tagawa; T Takubo; K Tanaka; T Nakao; Y Higeno; K Tamura; M Shimaoka; A Fujii; M Higashihata; Y Yasui; T Kim; A Hiraoka; N Tatsumi
Journal:  J Clin Microbiol       Date:  1997-10       Impact factor: 5.948

6.  Evaluation of an automated sample preparation protocol for quantitative detection of hepatitis C virus RNA.

Authors:  Evelyn Stelzl; Andrea Kormann-Klement; Josef Haas; Elisabeth Daghofer; Brigitte I Santner; Egon Marth; Harald H Kessler
Journal:  J Clin Microbiol       Date:  2002-04       Impact factor: 5.948

Review 7.  Automation of laboratory testing for infectious diseases using the polymerase chain reaction-- our past, our present, our future.

Authors:  D Jungkind
Journal:  J Clin Virol       Date:  2001-01       Impact factor: 3.168
  7 in total
  2 in total

1.  Genotype 3 diversity and quantification of hepatitis E virus RNA.

Authors:  Florence Abravanel; Karine Sandres-Saune; Sebastien Lhomme; Martine Dubois; Jean-Michel Mansuy; Jacques Izopet
Journal:  J Clin Microbiol       Date:  2012-01-11       Impact factor: 5.948

2.  Evaluation of the COBAS AmpliPrep-total nucleic acid isolation-COBAS TaqMan hepatitis B virus (HBV) quantitative test and comparison to the VERSANT HBV DNA 3.0 assay.

Authors:  Christophe Ronsin; Anne Pillet; Corinne Bali; Gérard-Antoine Denoyel
Journal:  J Clin Microbiol       Date:  2006-04       Impact factor: 5.948
  2 in total

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