Literature DB >> 15297535

Comparison of sequence analysis and a novel discriminatory real-time PCR assay for detection and quantification of Lamivudine-resistant hepatitis B virus strains.

Fiona Wightman1, Tomos Walters, Anna Ayres, Scott Bowden, Angeline Bartholomeusz, Daryl Lau, Stephen Locarnini, Sharon R Lewin.   

Abstract

We report a rapid and accurate real-time PCR-based method to quantify wild-type and lamivudine-resistant hepatitis B virus by using a common forward primer paired with different reverse primers. Excellent concordance was demonstrated between sequencing and the discriminatory real-time assay; however, a mixture of quasispecies was more frequently detected by discriminatory real-time PCR.

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Year:  2004        PMID： 15297535      PMCID： PMC497642          DOI： 10.1128/JCM.42.8.3809-3812.2004

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  15 in total

Review 1.  Nomenclature for antiviral-resistant human hepatitis B virus mutations in the polymerase region.

Authors:  L J Stuyver; S A Locarnini; A Lok; D D Richman; W F Carman; J L Dienstag; R F Schinazi
Journal:  Hepatology       Date:  2001-03       Impact factor: 17.425

2.  Line probe assay for monitoring drug resistance in hepatitis B virus-infected patients during antiviral therapy.

Authors:  L Stuyver; C Van Geyt; S De Gendt; G Van Reybroeck; F Zoulim; G Leroux-Roels; R Rossau
Journal:  J Clin Microbiol       Date:  2000-02       Impact factor: 5.948

Review 3.  Resistance of hepatitis B virus to antiviral drugs: current aspects and directions for future investigation.

Authors:  W E Delaney; S Locarnini; T Shaw
Journal:  Antivir Chem Chemother       Date:  2001-01

4.  The complete nucleotide sequence of the cloned DNA of hepatitis B virus subtype adr in pADR-1.

Authors:  R B Gan; M J Chu; L P Shen; S W Qian; Z P Li
Journal:  Sci Sin B       Date:  1987-05

5.  Effects of primer-template mismatches on the polymerase chain reaction: human immunodeficiency virus type 1 model studies.

Authors:  S Kwok; D E Kellogg; N McKinney; D Spasic; L Goda; C Levenson; J J Sninsky
Journal:  Nucleic Acids Res       Date:  1990-02-25       Impact factor: 16.971

6.  Use of mutator cells as a means for increasing production levels of a recombinant antibody directed against Hepatitis B.

Authors:  G Coia; A Ayres; G G Lilley; P J Hudson; R A Irving
Journal:  Gene       Date:  1997-11-12       Impact factor: 3.688

7.  Analysis of hepatitis B viral load decline under potent therapy: complex decay profiles observed.

Authors:  S R Lewin; R M Ribeiro; T Walters; G K Lau; S Bowden; S Locarnini; A S Perelson
Journal:  Hepatology       Date:  2001-11       Impact factor: 17.425

8.  Identification of a new variant in the YMDD motif of the hepatitis B virus polymerase gene selected during lamivudine therapy.

Authors:  Hubert G M Niesters; Robert A DE Man; Suzan D Pas; Edwin Fries; Albert D M E Osterhaus
Journal:  J Med Microbiol       Date:  2002-08       Impact factor: 2.472

9.  Resistance to adefovir dipivoxil therapy associated with the selection of a novel mutation in the HBV polymerase.

Authors:  Peter Angus; Rhys Vaughan; Shelly Xiong; Huiling Yang; William Delaney; Craig Gibbs; Carol Brosgart; Danielle Colledge; Rosalind Edwards; Anna Ayres; Angeline Bartholomeusz; Stephen Locarnini
Journal:  Gastroenterology       Date:  2003-08       Impact factor: 22.682

10.  Monitoring drug resistance in chronic hepatitis B virus (HBV)-infected patients during lamivudine therapy: evaluation of performance of INNO-LiPA HBV DR assay.

Authors:  Anna S F Lok; Fabien Zoulim; Stephen Locarnini; Alessandra Mangia; Grazia Niro; Hilde Decraemer; Geert Maertens; Frank Hulstaert; Karen De Vreese; Erwin Sablon
Journal:  J Clin Microbiol       Date:  2002-10       Impact factor: 5.948
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  6 in total

1.  Comparison of amplicon-sequencing, pyrosequencing and real-time PCR for detection of YMDD mutants in patients with chronic hepatitis B.

Authors:  Zhi-Jun Yang; Mei-Zeng Tu; Jian Liu; Xiao-Ling Wang; Hong-Zhi Jin
Journal:  World J Gastroenterol       Date:  2006-11-28       Impact factor: 5.742

Review 2.  Real-time PCR in clinical microbiology: applications for routine laboratory testing.

Authors:  M J Espy; J R Uhl; L M Sloan; S P Buckwalter; M F Jones; E A Vetter; J D C Yao; N L Wengenack; J E Rosenblatt; F R Cockerill; T F Smith
Journal:  Clin Microbiol Rev       Date:  2006-01       Impact factor: 26.132

3.  Detection of YMDD mutants using universal template real-time PCR.

Authors:  Rong-Sheng Wang; Hua Zhang; Yu-Fen Zhu; Bei Han; Zhi-Jun Yang
Journal:  World J Gastroenterol       Date:  2006-02-28       Impact factor: 5.742

4.  Ultrasensitive genotypic detection of antiviral resistance in hepatitis B virus clinical isolates.

Authors:  Jie Fang; Michael J Wichroski; Steven M Levine; Carl J Baldick; Charles E Mazzucco; Ann W Walsh; Bernadette K Kienzle; Ronald E Rose; Kevin A Pokornowski; Richard J Colonno; Daniel J Tenney
Journal:  Antimicrob Agents Chemother       Date:  2009-05-11       Impact factor: 5.191

5.  Establishment of real time allele specific locked nucleic acid quantitative PCR for detection of HBV YIDD (ATT) mutation and evaluation of its application.

Authors:  Yongbin Zeng; Dezhong Li; Wei Wang; Mingkuan Su; Jinpiao Lin; Huijuan Chen; Ling Jiang; Jing Chen; Bin Yang; Qishui Ou
Journal:  PLoS One       Date:  2014-02-28       Impact factor: 3.240

Review 6.  Detection and monitoring of virus infections by real-time PCR.

Authors:  F Watzinger; K Ebner; T Lion
Journal:  Mol Aspects Med       Date:  2006-02-14
  6 in total

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